Induction of pluripotent stem cells from adult human fibroblasts by defined factors
Induction of pluripotent stem cells from adult human fibroblasts by defined factors
复制标题
DOI：
10.1016/j.cell.2007.11.019
复制
发表时间：
2007-11-30
期刊：
影响因子：
64.5
通讯作者：
Yamanaka, Shinya
中科院分区：
文献类型：
--
作者：
Takahashi, Kazutoshi;Tanabe, Koji;Yamanaka, Shinya
成功地将分化的人类体细胞重编程为多能状态将允许产生患者和疾病特异性干细胞。我们以前报道的诱导多能干细胞（iPS）的产生，能够生殖系传输，从小鼠体细胞的四个定义的转录因子转导。在这里，我们证明了从成人皮肤成纤维细胞中产生iPS细胞，具有相同的四个因子：Oct 3/4，Sox 2，Klf 4和c-Myc。人iPS细胞在形态、增殖、表面抗原、基因表达、多能细胞特异性基因的表观遗传状态和端粒酶活性方面与人胚胎干（ES）细胞相似。此外，这些细胞在体外和畸胎瘤中可以分化为三个胚层的细胞类型。这些发现表明，iPS细胞可以从成人成纤维细胞产生。
Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.