Herpes simplex virus latency analyzed by in situ hybridization.
Herpes simplex virus latency analyzed by in situ hybridization.
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通过原位杂交分析单纯疱疹病毒潜伏期。
DOI：
10.1007/978-3-642-74425-9_1
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发表时间：
1989
影响因子：
--
通讯作者：
Stevens,JG
中科院分区：
文献类型：
--
作者：
Stevens,JG
迄今为止，原位核酸杂交技术已被证明在体内产生有关潜伏性单纯疱疹病毒（HSV）感染的基本特性的信息方面非常有用。在采用现有方法检测病毒转录本的过程中，已经收集到令人信服的数据，这些数据可以确定(a)建立潜伏感染的细胞，以及(b)潜伏感染细胞中存在的病毒转录本的性质。这些正在进行的描述性研究为研究潜伏感染的潜在机制提供了必要的见解和理论框架。除了对潜伏感染细胞的研究外，一些实验室还采用原位方法来研究急性感染的发病机制，在很大程度上证实了早先从经典方法推断出的结论。本章重点介绍了最近在潜伏期分析方面取得的成功，这是由重组DNA技术的融合所实现的，重组DNA技术可以在病毒基因组上产生具有明确极性和物理位置的单链探针，以及越来越敏感的原位杂交技术。
To date, in situ nucleic acid hybridization technology has proved singularly useful in generating information about the basic properties of latent herpes simplex virus (HSV) infections in vivo. In adapting extant methods to detect viral transcripts, convincing data have been gathered which identify (a) the cell in which the latent infection is established, and (b) the nature of viral transcripts present in latently infected cells. These ongoing descriptive studies provide the necessary insight and theoretical framework for investigations into the underlying mechanisms involved in latent infection. In addition to studies of latently infected cells, in situ methodology has been employed in several laboratories to investigate the pathogenesis of acute infection, largely confirming the conclusions earlier inferred from classical methods. This chapter focuses on the recent successes in analyses of latency made possible by the convergence of recombinant DNA technology, which generates single stranded probes of defined polarity and physical location on the viral genome, and increasingly sensitive techniques for in situ hybridization.