Archaebacterial ATPases: relationship to other ion-translocating ATPase families examined in terms of immunological cross-reactivity.
Archaebacterial ATPases: relationship to other ion-translocating ATPase families examined in terms of immunological cross-reactivity.
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古细菌 ATP 酶：根据免疫交叉反应性检查与其他离子转位 ATP 酶家族的关系。
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
Y. Mukohata
中科院分区：
文献类型：
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作者：
J. Konishi;K. Denda;T. Oshima;T. Wakagi;E. Uchida;Y. Ohsumi;Y. Anraku;T. Matsumoto;T. Wakabayashi;Y. Mukohata
用免疫印迹法研究了3种古细菌H（+）-ATP酶，即嗜热菌PS 3（TF 1）的F1-ATP酶和酿酒酵母（Saccharomycescerevisiae）的液泡膜ATP酶之间的免疫交叉反应性。三种古细菌ATP酶的免疫交叉反应性非常相似，表明它们属于同一个ATP酶家族。三种古细菌之一酸热硫化叶菌的ATP酶与TF 1之间也有交叉反应。S.酿酒酵母液泡ATP酶与针对三种古细菌ATP酶中的每一种制备的抗体反应，但不与针对TF 1的抗体反应。电镜观察表明，硫化叶菌ATP酶的寡聚体结构与F1-ATP酶的结构非常相似。这些结果，综合起来，表明古细菌ATP酶共享密切的结构相似性与液泡ATP酶，并在较小的程度上，与F0 F1-ATP酶。
Immunological cross-reactivity among three types of H(+)-ATPases, that is, three archaebacterial ATPases, the F1-ATPase from thermophilic bacterium PS3 (TF1) and the vacuolar membrane ATPase from Saccharomyces cerevisiae, was examined by means of immunoblot analyses. The three archaebacterial ATPases were very similar in immunological cross-reactivity, suggesting that they belong to the same family of ATPases. Cross-reaction was also observed between the ATPase from Sulfolobus acidocaldarius, one of the three archaebacteria, and TF1. S. cerevisiae vacuolar ATPase reacted with the antibodies prepared against each of the three archaebacterial ATPases, but did not react with the antibody against TF1. Electron microscopic examination revealed that the oligomeric structure of Sulfolobus ATPase was very similar to that of F1-ATPase. These results, taken together, suggest that the archaebacterial ATPases share close structural similarities with the vacuolar ATPases, and, to a lesser degree, with the F0F1-ATPases.