Runx1 is involved in the fusion of the primary and the secondary palatal shelves
Runx1 is involved in the fusion of the primary and the secondary palatal shelves
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DOI：
10.1016/j.ydbio.2008.10.018
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发表时间：
2009-02-15
影响因子：
2.7
通讯作者：
Yamashiro, Takashi
中科院分区：
文献类型：
--
作者：
Charoenchaikorn, Kesinee;Yokomizo, Tomomasa;Yamashiro, Takashi
Runx1在腭架的内侧缘上皮细胞中表达。复苏的Runx1（-/-）小鼠在初级和次级腭架之间的前部连接处显示出有限的裂开，但在次级腭之间的连接处没有。在野生型小鼠中，融合的上皮表面表现出圆形鹅卵石样外观，而这种细胞突起在Runx1突变体中不太明显。我们还发现，FGF18表达的间充质中的潜在的腭和局部应用的FGF18诱导异位Runx1表达的上皮细胞的腭外植体，表明Runx1诱导间充质FGF18信号。另一方面，不成对的腭外植体培养揭示了前-后（A-P）差异的存在，在移植和融合机制。有趣的是，Runx1突变体中前裂的位置对应于具有不同突变行为的区域。这些数据表明，Runx1在腭融合中的细胞形态学变化中的一种新功能，至少部分是。通过上皮间充质相互作用由间充质Fgf信号传导调节。(c)2008年爱思唯尔公司All rights reserved.
Runx1 is expressed in medial edge epithelial (MEE) cells of the palatal shelf. Conditionally rescued Runx1(-/-) mice showed limited clefting in the anterior junction between the primary and the secondary palatal shelves, but not in the junction between the secondary palates. In wild type mice, the fusing epithelial surface exhibited a rounded cobblestone-like appearance, while such cellular prominence was less evident in the Runx1 mutants. We also found that Fgf18 was expressed in the mesenchyme underlying the MEE and that locally applied FGF18 induced ectopic Runx1 expression in the epithelium of the palatal explants, indicating that Runx1 was induced by mesenchymal Fgf18 signaling. On the other hand, unpaired palatal explant cultures revealed the presence of anterior-posterior (A-P) differences in the MEE fates and fusion mechanism. Interestingly, the location of anterior clefting in Runx1 mutants corresponded to the region with different MEE behavior. These data showed a novel function of Runx1 in morphological changes in the MEE cells in palatal fusion, which is, at least in part. regulated by the mesenchymal Fgf signaling via an epithelial mesenchymal interaction. (c) 2008 Elsevier Inc. All rights reserved.