Comparative analysis of mesenchymal stromal cells from different tissue sources in respect to articular cartilage tissue engineering
Comparative analysis of mesenchymal stromal cells from different tissue sources in respect to articular cartilage tissue engineering
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DOI：
10.4149/gpb_2015044
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发表时间：
2016-04-01
影响因子：
1.5
通讯作者：
Varga, Ivan
中科院分区：
文献类型：
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作者：
Danisovic, L'ubos;Bohac, Martin;Varga, Ivan
本研究的主要目的是比较从骨髓、脂肪组织和脐带中获得的间充质基质细胞（MSCs）在关节软骨再生方面的生物学特性。分离骨髓间充质干细胞并在体外扩增至第三代。通过细胞分析仪CEDEX XS分析增殖动力学，并通过流式细胞术评估所选标志物的表达。用倒置显微镜和透射电镜分析了其形貌。使用含有TGF-β 1的颗粒培养系统和软骨形成培养基来诱导软骨形成分化。通过实时PCR分析软骨发生;比较I型和II型胶原的表达。来自所有来源的MSC显示出相似的增殖动力学和共享的CD 73、CD 90和CD 105表达;并且对CD 14、CD 20、CD 34和CD 45呈阴性。倒置显微镜和透射电镜观察显示，所有分析的MSC的形态相似。来自所有来源的细胞经历软骨分化-它们表达透明软骨典型的II型胶原和酸性粘多糖。在所获得的结果的基础上，应该强调的是，来自骨髓、脂肪组织和脐带的MSC具有相同的生物学特性。它们具有成软骨潜能，可用于软骨组织工程。
The main goal of this study was a comparison of biological properties of mesenchymal stromal cells (MSCs) obtained from bone marrow, adipose tissue and umbilical cord with respect to articular cartilage regeneration. MSCs were isolated and expanded in vitro up to the third passage. The kinetics of proliferation was analyzed by cell analyzer CEDEX XS and expression of selected markers was assessed by flow cytometry. The morphology was analyzed by inverted microscope and TEM. Pellet culture system and chondrogenic medium containing TGF-beta 1 was used to induce chondrogenic differentiation. Chondrogenesis was analyzed by real-time PCR; the expression of collagen type I and type II was compared. MSCs from all sources showed similar kinetics of proliferation and shared expression of CD73, CD90 and CD105; and were negative for CD14, CD20, CD34 and CD45. Observation under inverted microscope and TEM showed similar morphology of all analyzed MSCs. Cells from all sources underwent chondrogenic differentiation - they expressed collagen type II and acid mucopolysaccharides typical for hyaline cartilage. On the basis of obtained results it should be emphasized that MSCs from bone marrow, adipose tissue and umbilical cord share biological properties. They possess the chondrogenic potential and may be utilized in cartilage tissue engineering.