Eucalyptol alleviates inflammation and pain responses in a mouse model of gout arthritis
Eucalyptol alleviates inflammation and pain responses in a mouse model of gout arthritis
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桉树脑可减轻痛风关节炎小鼠模型的炎症和疼痛反应
DOI：
10.1111/bph.14967
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发表时间：
2020-02-12
影响因子：
7.3
通讯作者：
Liu, Boyi
中科院分区：
文献类型：
--
作者：
Yin, Chengyu;Liu, Boyu;Liu, Boyi
背景与目的痛风性关节炎是由尿酸盐（MSU）结晶在关节及关节周围组织积聚引起的，引起剧烈疼痛并影响患者的生活质量。桉叶醇（1，8-桉叶醇）是桉叶精油中的主要成分，已知具有抗炎和镇痛特性。本研究旨在探讨杜仲醇对痛风性关节炎的治疗作用及其相关机制。实验方法通过小鼠踝关节注射MSU建立痛风性关节炎模型。踝关节水肿，机械性异常性疼痛，中性粒细胞浸润，氧化应激，NLRP 3炎性小体，和TRPV 1的expressioned.Key结果桉树脑衰减MSU诱导的机械性异常性疼痛和踝关节水肿的剂量依赖性，有效性类似于吲哚美辛。桉树脑减少踝关节组织中的炎性细胞浸润。在体内，桉叶醇抑制了由MSU诱导的踝关节组织中的NLRP 3炎性体活化和促炎细胞因子产生。桉树脑能降低MSU在体外RAW264.7细胞和体内踝关节组织中诱导的氧化应激，表现为抗氧化酶活性的增加和ROS的减少。桉叶醇减弱MSU诱导的踝关节组织和支配踝关节的背根神经节神经元TRPV 1表达上调。杜仲醇对踝关节水肿，机械异常性疼痛，NLRP 3炎性小体，IL-1 β，和TRPV 1的表达的体内效果进行了模拟治疗MSU注射小鼠与antioxidants.Conclusion and Implications杜仲醇通过可能涉及抗氧化作用的机制来抑制MSU诱导的疼痛和炎症。桉树脑和其他抗氧化剂可能代表痛风关节炎有前途的治疗选择。
Background and Purpose Gout arthritis, which is provoked by monosodium urate (MSU) crystal accumulation in the joint and periarticular tissues, induces severe pain and affects quality of life of the patients. Eucalyptol (1,8-cineol), the principal component in the essential oils of eucalyptus leaves, is known to possess anti-inflammatory and analgesic properties. We aimed to examine the therapeutic effects of eucalyptol on gout arthritis and related mechanisms.Experimental Approach A mouse model of gout arthritis was established via MSU injection into the ankle joint. Ankle oedema, mechanical allodynia, neutrophil infiltration, oxidative stress, NLRP3 inflammasome, and TRPV1 expression were examined.Key Results Eucalyptol attenuated MSU-induced mechanical allodynia and ankle oedema in dose-dependently, with effectiveness similar to indomethacin. Eucalyptol reduced inflammatory cell infiltrations in ankle tissues. Eucalyptol inhibited NLRP3 inflammasome activation and pro-inflammatory cytokine production induced by MSU in ankle tissues in vivo. Eucalyptol reduced oxidative stress induced by MSU in RAW264.7 cells in vitro as well as in ankle tissues in vivo, indicated by an increase in activities of antioxidant enzymes and reduction of ROS. Eucalyptol attenuated MSU-induced up-regulation of TRPV1 expression in ankle tissues and dorsal root ganglion neurons innervating the ankle. The in vivo effects of eucalyptol on ankle oedema, mechanical allodynia, NLRP3 inflammasome, IL-1 beta, and TRPV1 expression were mimicked by treating MSU-injected mice with antioxidants.Conclusion and Implications Eucalyptol alleviates MSU-induced pain and inflammation via mechanisms possibly involving anti-oxidative effect. Eucalyptol and other antioxidants may represent promising therapeutic options for gout arthritis.