HOP STUNT VIROID - MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE COMPLETE CDNA COPY
HOP STUNT VIROID - MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE COMPLETE CDNA COPY
复制标题
DOI：
10.1093/nar/11.18.6185
复制
发表时间：
1983-01-01
影响因子：
14.9
通讯作者：
OKADA, Y
中科院分区：
文献类型：
--
作者：
OHNO, T;TAKAMATSU, N;OKADA, Y
通过冈山和贝格的方法克隆了啤酒花矮化类病毒（HSV）的完整cDNA（Mol.Cell.Biol.2，161 -170.（1982）），并且已经建立了完整的核苷酸序列。共价闭合的环状单链HSV RNA由297个核苷酸组成。预测的HSV二级结构包含67%的碱基配对残基。天然HSV可具有先前建立的类病毒特征的延伸的棒状结构。天然HSV的中心区域具有与迄今为止测序的所有类病毒中发现的保守区域相似的结构，除了鳄梨晒斑类病毒。与Ula RNA的5′端同源的序列也存在于HSV的序列中，但在中央保守区没有。
The complete cDNA of hop stunt viroid (HSV) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161–170.(1982)) and the complete nucleotide sequence has been established. The covalently closed circular single-stranded HSV RNA consists of 297 nucleotides. The secondary structure predicted for HSV contains 67% of its residues base-paired. The native HSV can possess an extended rod-like structure characteristic of viroids previously established. The central region of the native HSV has a similar structure to the conserved region found in all viroids sequenced so far except for avocado sunblotch viroid. The sequence homologous to the 5′-end of Ula RNA is also found in the sequence of HSV but not in the central conserved region.