An Artificial Crossing Technique for Selaginella
An Artificial Crossing Technique for Selaginella
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卷柏人工杂交技术
DOI：
10.2307/1546905
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发表时间：
1979
影响因子：
0.8
通讯作者：
T. R. Webster
中科院分区：
文献类型：
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作者：
T. R. Webster
已经描述了获得卷柏属的小配子体、大配子体和孢子的各种方法。Slagg（1932）在S. kraussiana（Kunze）A. Br.，在巴黎砖的灰泥上发芽的小孢子。Bold（1967）描述了一种使小孢子和大孢子萌发的类似方法。Bierhorst（1964）描述了用于课堂使用的获得卷柏属生殖阶段的方法。韦伯斯特（1967）描述了在温室和田间条件下孢子的诱导。Wetmore和Morel（1951）在S. Pallescens（Presl）Spring in Mart.和S.扇叶藻（L.）在无菌条件下在营养琼脂上春季。通过使用补充有各种生长因子的培养基，他们能够使配子体组织生长数月。尽管有上述方法可以使卷柏属的孢子萌发并获得有性生殖材料，但在该属中只有一个人工杂交的报道。Burgeff和Filippi（1957）在S.马氏泉变种martensii和S.马氏变种他们将表面灭菌的大孢子和小孢子一起播种在培养管中的营养琼脂上，在配子体形成后，用水淹没培养物以实现受精。30-40天后，出现孢子。利用这种方法，他们研究了S.马氏变种杂色的本文介绍了一种在几个方面不同于Burgeff和Filippi（1957）所用的杂交技术。虽然Burgeff和Filippi已成功地将其用于重复杂交，但该技术是为与S. kraussiana及其变种，下面的描述是基于对这些分类群的研究。应用该技术研究了猪色素沉着的遗传。克劳西亚纳湾金叶菊公牛将是稍后报告的主题。
Various methods for obtaining microgametophytes, megagametophytes, and sporelings of Selaginella have been described. Slagg (1932), in a study of microgametophyte development in S. kraussiana (Kunze) A. Br., germinated microspores on plaster of Paris blocks. A similar method for germinating both microspores and megaspores was described by Bold (1967). Bierhorst (1964) described methods for obtaining reproductive stages of Selaginella for classroom use. Webster (1967) described the induction of sporelings under greenhouse and field conditions. Wetmore and Morel (1951) germinated megaspores of S. pallescens (Presl) Spring in Mart. and S. flabellata (L.) Spring on nutrient agar under sterile conditions. Through the use of a medium supplemented with various growth factors, they were able to grow gametophytic tissue for several months. Despite the above procedures for germinating spores and obtaining sexual material of Selaginella, there is but one report of artificial crosses in the genus. Burgeff and Filippi (1957) made crosses between S. martensii Spring var. martensii and S. martensii var. variegata Hort. They sowed surface-sterilized megaspores and microspores together on nutrient agar in culture tubes and, after gametophytes had formed, flooded the cultures with water to achieve fertilization. After 30-40 days, sporelings appeared. Using this method, they studied the inheritance of variegation in S. martensii var. variegata. In this paper, a crossing technique differing in several respects from the one used by Burgeff and Filippi (1957) is described. Although it has been used successfully to repeat the crosses by Burgeff and Filippi, the technique was developed for making crosses with S. kraussiana and its varieties, and the following description is based on work with these taxa. Application of this technique to a study of inheritance of pigmentation in S. kraussiana var. aurea W. Bull will be the subject of a later report.