Fish-oil-derived n-3 polyunsaturated fatty acids reduce NLRP3 inflammasome activity and obesity-related inflammatory cross-talk between adipocytes and CD11b+ macrophages
Fish-oil-derived n-3 polyunsaturated fatty acids reduce NLRP3 inflammasome activity and obesity-related inflammatory cross-talk between adipocytes and CD11b+ macrophages
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DOI：
10.1016/j.jnutbio.2016.04.004
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发表时间：
2016-08-01
影响因子：
5.6
通讯作者：
Robinson, Lindsay E.
中科院分区：
文献类型：
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作者：
De Boer, Anna A.;Monk, Jennifer M.;Robinson, Lindsay E.
脂肪细胞-巨噬细胞串扰在肥胖脂肪组织（AT）中传播免疫应答。长链n-3多不饱和脂肪酸（LC n-3 PUFA）减轻炎症，部分通过上调脂联素;然而，具体机制尚不清楚。我们确定了饮食LC n-3 PUFA是否可以减轻脂肪细胞-巨噬细胞串扰，以及这是否依赖于脂联素介导的信号传导。我们利用体外共培养模型模拟肥胖AT中脂肪细胞：巨噬细胞的比例，其中3 T3-L1脂肪细胞与来自喂食高脂肪对照的C57 BL/6小鼠的脾CD 11b（+）巨噬细胞共培养（HF-CON; 34% w/w脂肪）或鱼油饲料（HF-FO;含有7.6%w/w FO的34%w/w脂肪）以及饲喂低脂对照的小鼠（LF-CON; 10%w/w脂肪）或FO饮食（LF-FO;含有3%w/w FO的10%w/w脂肪）。共培养条件测试了可溶性介体驱动机制（trans-well系统）、细胞接触和模拟急性或慢性炎症状况的低剂量脂多糖（LPS）的作用。与HF-CON巨噬细胞相比，来自急性LPS刺激的跨孔共培养的HF-FO巨噬细胞的Caspase 1、IL 1 β和IL 18 mRNA表达以及细胞caspase-1活性降低（P
Adipocyte-macrophage cross-talk propagates immune responses in obese adipose tissue (AT). Long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) mitigate inflammation, partly through up-regulation of adiponectin; however, specific mechanisms are unclear. We determined if adipocyte-macrophage crosstalk could be mitigated by dietary LC n-3 PUFA and if this was dependent on adiponectin-mediated signaling. We utilized an in vitro co-culture model mimicking the ratio of adipocytes:macrophages in obese AT, whereby 3T3-L1 adipocytes were co-cultured with splenic CD11b(+) macrophages from C57BL/6 mice fed high fat control (HF-CON; 34% w/w fat) or fish oil diets (HF-FO; 34% w/w fat containing 7.6% w/w FO), as well as mice fed low-fat control (LF-CON; 10% w/w fat) or FO diets (LF-FO; 10% w/w fat containing 3% w/w FO). Co-culture conditions tested effects of soluble mediator-driven mechanisms (trans-well system), cell contact and low-dose lipopolysaccharide (LPS) mimicking acute or chronic inflammatory conditions. HF-FO macrophages from acute LPS-stimulated trans-well co-cultures had decreased mRNA expression of Casp1, Il1 beta and Il18, as well as cellular caspase-1 activity compared to HF-CON macrophages (P