Serratia ureilytica sp nov., a novel urea-utilizing species
Serratia ureilytica sp nov., a novel urea-utilizing species
复制标题
DOI：
10.1099/ijs.0.63674-0
复制
发表时间：
2005-09-01
影响因子：
2.8
通讯作者：
Chakraborty, R
中科院分区：
文献类型：
--
作者：
Bhadra, B;Roy, P;Chakraborty, R
从印度西孟加拉Jalpaiguri区Hasimara的Torsa河中分离到一种革兰氏阴性、杆状、尿素溶解和不形成孢子的细菌，命名为菌株尼瓦51（T）。根据16 S rRNA基因序列相似性分析，菌株尼瓦51（T）属于γ-变形菌门，与粘质沙雷氏菌（Serratia marcescens subsp.）sakuensis（98- 35%）和S.粘质虫亚种marcescens（98- 30%）;然而，菌株尼瓦51（T）与S. marcescens的DNA-DNA杂交。该分离物基因组DNA的G + C含量为60mol%。菌株尼瓦51（T）的生化特性和脂肪酸分析结果均支持菌株属于沙雷氏菌属。此外，发现菌株尼瓦51（T）利用尿素作为氮源。DNA-DNA杂交及生理生化试验结果表明，菌株尼瓦51（T）与已知的沙雷氏菌属菌株具有明显的基因型和表型差异。因此，菌株尼瓦51（T）代表一个新的物种，建议将其命名为解脲沙雷氏菌新种，模式菌株尼瓦51（T）（= LIVIG 22860（T）= CCUG 50595（T））。
A Gram-negative, rod-shaped, urea-dissolving and non-spore-forming bacterium, designated strain NiVa 51(T), was isolated from water of the River Torsa in Hasimara, Jalpaiguri district, West Bengal, India. On the basis of 16S rRNA gene sequence similarity, strain NiVa 51(T) was shown to belong to the gamma-Proteobacteria and to be related to Serratia marcescens subsp. sakuensis (98-35 %) and S. marcescens subsp. marcescens (98-30 %); however, strain NiVa 51(T) exhibited only 43-7 % similarity to S. marcescens by DNA-DNA hybridization. The G + C content of the genomic DNA of the isolate was 60 mol%. Both biochemical characteristics and fatty acid analysis data supported the affiliation of strain NiVa 51(T) to the genus Serratia. Furthermore, strain NiVa 51(T) was found to utilize urea as nitrogen source. The results of DNA-DNA hybridization as well as physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain NiVa 51(T) from recognized Serratia species. Strain NiVa 51(T) therefore represents a novel species, for which the name Serratia ureilytica sp. nov. is proposed, with type strain NiVa 51(T) (= LIVIG 22860(T)= CCUG 50595(T)).