Theaflavin ameliorates cerebral ischemia-reperfusion injury in rats through its anti-inflammatory effect and modulation of STAT-1.
Theaflavin ameliorates cerebral ischemia-reperfusion injury in rats through its anti-inflammatory effect and modulation of STAT-1.
复制标题
DOI：
10.1155/mi/2006/30490
复制
发表时间：
2006
影响因子：
4.6
通讯作者：
Chen, Jin-He
中科院分区：
文献类型：
--
作者：
Cai, Fei;Li, Cai-Rong;Wu, Ji-Liang;Chen, Jian-Guo;Liu, Chao;Min, Qing;Yu, Wei;Ouyang, Chang-Han;Chen, Jin-He
茶黄素是红茶的主要成分，具有抗氧化、抗病毒、抗炎等生物学功能。本研究的目的是验证茶黄素是否可以减轻大脑中动脉闭塞（MCAO）大鼠模型的局灶性脑缺血损伤。将雄性 Sprague-Dawley 大鼠麻醉并进行 2 小时的 MCAO，然后再灌注 24 小时。茶黄素给药（5、10 和 20 mg/kg，静脉注射）可改善梗塞和水肿体积。茶黄素抑制损伤脑中白细胞浸润以及 ICAM-1、COX-2 和 iNOS 的表达。 STAT-1（一种介导细胞内向细胞核发出信号的蛋白质）的磷酸化比假手术组增强了 2 倍，并被茶黄素抑制。我们的研究表明，茶黄素通过限制白细胞浸润和 ICAM-1 的表达，并至少部分通过减少 STAT-1 的磷酸化来抑制缺血脑中炎症相关促氧化酶（iNOS 和 COX-2）的上调，从而显着保护神经元免受脑缺血再灌注损伤。
Theaflavin, a major constituent of black tea, possesses biological functions such as the antioxidative, antiviral, and anti-inflammatory ones. The purpose of this study was to verify whether theaflavin reduces focal cerebral ischemia injury in a rat model of middle cerebral artery occlusion (MCAO). Male Sprague-Dawley rats were anesthetized and subjected to 2 hours of MCAO followed 24 hours reperfusion. Theaflavin administration (5, 10, and 20 mg/kg, IV) ameliorated infarct and edema volume. Theaflavin inhibited leukocyte infiltration and expression of ICAM-1, COX-2, and iNOS in injured brain. Phosphorylation of STAT-1, a protein which mediates intracellular signaling to the nucleus, was enhanced 2-fold over that of sham group and was inhibited by theaflavin. Our study demonstrated that theaflavin significantly protected neurons from cerebral ischemia-reperfusion injury by limiting leukocyte infiltration and expression of ICAM-1, and suppressing upregulation of inflammatory-related prooxidative enzymes (iNOS and COX-2) in ischemic brain via, at least in part, reducing the phosphorylation of STAT-1.