Functional analysis of the Fusarium graminearum phosphatome
Functional analysis of the Fusarium graminearum phosphatome
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禾谷镰刀菌磷酸酶的功能分析
DOI：
10.1111/nph.13374
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发表时间：
2015-07-01
期刊：
影响因子：
9.4
通讯作者：
Ma, Zhonghua
中科院分区：
文献类型：
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作者：
Yun, Yingzi;Liu, Zunyong;Ma, Zhonghua
磷酸酶在真核生物的各种细胞过程中起着重要的调节作用。然而，在植物病原真菌的磷酸酶组的系统表征还没有报道。小麦赤霉病菌Fusarium graminearum含有82种磷酸酶。本研究对各磷酸酶的生物学功能进行了研究。虽然11个磷酸酶基因似乎是必不可少的，其他71个磷酸酶基因的缺失突变体，获得和表征的变化，在15个表型，包括营养生长，营养反应和毒力。总体而言，63个磷酸酶基因的缺失导致至少一种测定的表型发生变化。有趣的是，四个基因（Fg06297，Fg03333，Fg03826和Fg07932）的缺失并没有显着影响菌丝生长，但导致毒力大幅下降。蛋白质印迹分析表明，三个磷酸酶（Fg10516，Fg03333和Fg12867）作为负调节丝裂原活化蛋白激酶信号通路。此外，我们首次发现，FgCdc14是生长抑制剂，但在核糖体生物合成中起着重要作用。总之，在真菌磷酸酶组的第一个功能表征中，在植物病原真菌禾谷镰刀菌中鉴定了对菌丝生长、发育、植物感染和次生代谢的各个方面都很重要的磷酸酶。
Phosphatases are known to play important roles in the regulation of various cellular processes in eukaryotes. However, systematic characterization of the phosphatome has not been reported in phytopathogenic fungi. The wheat scab fungus Fusarium graminearum contains 82 putative phosphatases. The biological functions of each phosphatase were investigated in this study. Although 11 phosphatase genes appeared to be essential, deletion mutants of the other 71 phosphatase genes were obtained and characterized for changes in 15 phenotypes, including vegetative growth, nutrient response and virulence. Overall, the deletion of 63 phosphatase genes resulted in changes in at least one of the phenotypes assayed. Interestingly, the deletion of four genes (Fg06297, Fg03333, Fg03826 and Fg07932) did not dramatically affect hyphal growth, but led to strongly reduced virulence. Western blot analyses showed that three phosphatases (Fg10516, Fg03333 and Fg12867) functioned as negative regulators of the mitogen-activated protein kinase signaling pathways. In addition, we found, for the first time, that FgCdc14 is dispensable for growth, but plays an important role in ribosome biogenesis. Overall, in this first functional characterization of the fungal phosphatome, phosphatases important for various aspects of hyphal growth, development, plant infection and secondary metabolism were identified in the phytopathogenic fungus F.graminearum.See also the Commentary by Jia and Tang