Plasma-induced grafted polymerization of acrylic acid and subsequent grafting of collagen onto polymer film as biomaterials
Plasma-induced grafted polymerization of acrylic acid and subsequent grafting of collagen onto polymer film as biomaterials
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DOI：
10.1016/0142-9612(95)00316-9
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发表时间：
1996-08-01
期刊：
影响因子：
14
通讯作者：
Kao, CY
中科院分区：
文献类型：
--
作者：
Lee, SD;Hsiue, GH;Kao, CY
采用等离子体引发接枝聚合的方法，将聚丙烯酸（pAA）引入到Ar等离子体处理的硅橡胶（SR）膜表面。胶原蛋白（III型）也连接到SR表面上的PAA的羧基通过一个双亚胺试剂，以获得SR的二级结构。SR的表面性能，其特征在于，通过ATR-FTIR，ESCA，接触角，和SEM。SR表面的生物相容性通过角膜上皮（CE）细胞的培养进行了评价。随后，在不同的反应条件下，SR表面获得了75-450 μ g cm（-2）的pAA; 3-12 μ g cm（-2）的胶原蛋白连接在SR的改性表面上。此外，利用ATR-FTIR和ESCA来证实这些反应的过程。用接触角仪测定了改性SR的结晶度。SR与pAA接枝的接触角值约为45-50度;随后在pAA-g-SR上获得50-55度的接触角以进一步与胶原连接。此外，还考察了表面性质对CE细胞在改性表面上迁移、生长和附着的影响。在此，未处理的SR用作对照。实验结果表明，控制SR上的CE细胞数量可以忽略不计。清楚地观察到细胞在PAA接枝表面上的附着，并出现伪足;然而，细胞生长受到抑制。这种抑制可能是由PAA接枝膜的酸性环境引起的。然而，细胞附着和生长到胶原蛋白连接的表面是显着的。此外，附着在该表面上的细胞的形态被认为是原代细胞的正常形态。在SR表面上引入的胶原蛋白没有变性，即保持胶原蛋白的天然性质。本研究所获得的结果将有望导致成功地开发用于临床应用的改良SR。(C)1996年爱思唯尔科学有限公司
Polyacrylic acid (pAA) was introduced onto Ar-plasma treatment silicone rubber (SR) membrane surfaces by plasma-induced grafted polymerization. Collagen (type III) was also linked with the carboxylic group of PAA grafted onto the SR surface via a carbodiimine agent to obtain a secondary structure of SR. The SR surface properties were characterized by ATR-FTIR, ESCA, contact angle, and SEM. The biocompatibility of the SR surface was evaluated by a culture of cornea epithelial (CE) cells. Subsequently, 75-450 mu g cm(-2) of pAA were obtained on the SR surfaces under different reactive conditions; 3-12 mu g cm(-2) of collagen were linked on modified surfaces of SR. Moreover, ATR-FTIR and ESCA were utilized to confirm the proceedings of these reactions. The hydrophility of the modified SR was measured by a contact angle meter. The values of contact angle for SR grafted with pAA were approximately 45-50 degrees; a 50-55 degrees contact angle on pAA-g-SR to be further linked with collagen was subsequently obtained. Moreover, the influence of surface properties toward migration, growth and attachment of CE cells on the modified surfaces was also examined. Here, untreated SR was used as a control. Experimental results indicated that the number of CE cells attached onto the controlled SR was negligible. The attach ment of cells onto pAA-grafted surfaces was clearly observed and peusopoda occurred; however, cell growth was depressed. This depression may have been caused by the acid environ ment of the pAA-grafted membrane. Nevertheless, both cell attachment and growth onto collagen-linked surfaces were significant. In addition, the morphology of the cells attached onto this surface was considered normal for primary cells. Collagen introduced on the SR surface was not denatured, i.e. the natural properties of collagen were maintained. The results obtained in this study will hopefully lead to the successful development of modified SR for clinical applications. (C) 1996 Elsevier Science Limited