Characteristics of sensory dorsal root ganglia neurons innervating the lumbar vertebral body in rats
Characteristics of sensory dorsal root ganglia neurons innervating the lumbar vertebral body in rats
复制标题
DOI：
10.1016/j.jpain.2007.01.004
复制
发表时间：
2007-06-01
期刊：
影响因子：
4
通讯作者：
Takahashi, Kazuhisa
中科院分区：
文献类型：
--
作者：
Ohtori, Seiji;Inoue, Gen;Takahashi, Kazuhisa
采用逆行神经转运法、凝集素亲和和免疫组化方法研究大鼠L5椎体感觉背根神经节（DRG）神经元的特征，以进一步阐明腰椎骨质疏松性骨折后部分老年患者背部、外侧干和髂嵴弥漫性疼痛的原因。我们用降钙素基因相关肽（CGRP）作为含小肽神经元的标记物，用结合Griffonia simplicifolia （IB4）分离素的糖蛋白作为不含小肽神经元的标记物。支配L5椎体的神经元，用氟金（FG）逆行标记，分布于从T13到L6的整个drg。cgrp免疫反应性（IR） fg标记神经元比例为32%。ib4结合的fg标记神经元比例明显较小，为4%。其他非cgrp - ir和非ib4结合的神经元大多是大神经元，它们可能从椎体传递本体感觉。传递疼痛的神经元多为含CGRIP-IR肽的神经元。它们可能在椎体痛觉中作为多肽性DRG神经元发挥更重要的作用。观点：本文表明椎体受CGRP-IR神经元支配。CGRP-IR神经元可能通过多肽性DRG神经元参与痛觉。这些发现有助于理解与椎体相关的疼痛，如肿瘤、感染或骨质疏松性骨折。(c) 2007年美国疼痛学会。
Characteristics of sensory dorsal root ganglia (DRG) neurons innervating the L5 vertebral body were investigated in rats by using a retrograde neurotransport method, lectin affinity- and immuno-histochemistry to further elucidate the causes of diffuse pain suffered by some elderly patients in their back, lateral trunk, and iliac crest, after lumbar osteoporotic vertebral fracture. We used calcitonin gene-related peptide (CGRP) as a marker of small pepticle-containing neurons and the glycoprotein binding the isolectin from Griffonia simplicifolia (IB4) as a marker of small non-peptide-containing neurons. Neurons innervating the L5 vertebral bodies, retrogradely labeled with fluorogold (FG), were distributed throughout DRGs from T13 to L6. The proportion of CGRP-immunoreactive (IR) FG-labeled neurons was 32%. The proportion of IB4-binding FG-labeled neurons was significantly smaller, at 4%. Other neurons that were non-CGRP-IR and non-IB4-binding were mostly large neurons, and they may transmit proprioception from vertebral bodies. Most neurons transmitting pain are CGRIP-IR peptide- containing neurons. They may have a more significant role in pain sensation in the vertebral bodies as peptidergic DRG neurons.Perspective: This article shows that,vertebral bodies are innervated by CGRP-IR neurons. CGRP-IR neurons may playa role in pain sensation through peptidergic DRG neurons. These findings contribute to an understanding of pain associated with the vertebral body such as tumor, infection, or osteoporotic fracture. (c) 2007 by the American Pain Society.