Positive Effects of Qing'e Pill on Trabecular Microarchitecture and its Mechanical Properties in Osteopenic Ovariectomised Mice
Positive Effects of Qing'e Pill on Trabecular Microarchitecture and its Mechanical Properties in Osteopenic Ovariectomised Mice
复制标题
清峨丸对骨质疏松去卵巢小鼠小梁微结构及其机械性能的积极影响。
DOI：
10.1007/s11655-016-2604-0
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发表时间：
2019-04-01
影响因子：
2.9
通讯作者：
Lu Lin
中科院分区：
文献类型：
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作者：
Shuai Bo;Zhu Rui;Lu Lin
目的探讨青娥丸（QEP）对绝经后骨质疏松症模型小鼠松质骨微结构的影响及其对β-catenin水平的影响。方法将96只8周龄无特定病原体的C57 BL/6小鼠随机分为4组（24只/组）：假手术组、去卵巢骨质疏松症模型组、雌二醇治疗组和QEP治疗组。手术后3个月，解剖动物的第三腰椎和左侧股骨，并使用微型计算机断层扫描（micro-CT）进行扫描，以获取其松质骨微观结构的三维（3D）参数。结果雌激素组和QEP干预组的骨体积分数、骨小梁数量、骨小梁厚度、骨表面积/骨体积比（BS/BV）和β-catenin表达均较模型组显著增加（P
ObjectiveTo investigate the impact of Qing'e Pill (QEP) on the cancellous bone microstructure and its effect on the level of -catenin in a mouse model of postmenopausal osteoporosis.MethodsNinety-six 8-week-old specific pathogen free C57BL/6 mice were randomly divided into 4 groups (24/group): sham, ovariectomised osteoporosis model, oestradiol-treated, and QEP-treated groups. Three months after surgery, the third lumbar vertebra and left femur of the animals were dissected and scanned using micro-computed tomography (micro-CT) to acquire three-dimensional (3D) parameters of their cancellous bone microstructure. The impact of ovariectomy, the effect of oestradiol and QEP intervention on cancellous bone microstructure, and the expression of -catenin were evaluated.ResultsThe oestradioland the QEP-treated groups exhibited a significant increase in the bone volume fraction, trabecular number, trabecular thickne ss s, bone surface to bone volume ratio (BS/BV), and -catenin expression compared with those of the model group (P