Development of serotoninergic chick retinal neurons
Development of serotoninergic chick retinal neurons
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DOI：
10.1016/s0736-5748(97)00026-9
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发表时间：
1997-10-01
影响因子：
1.8
通讯作者：
Saavedra, JP
中科院分区：
文献类型：
--
作者：
Rios, H;Brusco, A;Saavedra, JP
人们对发育中的视网膜中的许多神经递质进行了详细研究。几乎所有已知的神经递质和神经调节剂都通过甲醛诱导的荧光、摄取放射自显影或免疫组织化学程序在脊椎动物视网膜中得到证实。 5HT（5HT）无长突神经元位于视网膜内核层（INL），其树突在内丛状层（IPL）内扩散。目前的工作描述了从胚胎第 12 天到出生后第 7 天的小鸡视网膜中具有分层树突分支模式的血清素能无长突神经元的发育形态模式。还描述了血清素能双极神经元。 5HT-无长突神经元具有圆形或梨形的体细胞和初级树突树，其朝向贯穿 INL 的 IPL，显示出多个静脉曲张。然后次生树突穿过 INL，没有任何侧枝。在 IPL 的外部和内部边缘，初级和次级树突分别产生外部和内部血清素能网络。当初级树突树到达 IPL 时，它会在第 1 层（外部血清素能网络）中横向偏转。然后，三级分支从次级树突中产生，并在 IPL 的最内层（内部血清素能网络）中偏转。 5HT 无长突细胞的最终分支模式出现在胚胎第 14 天，并在孵化时完全发育。孵化时 INL 中也存在血清素能 (5HT) 双极神经元。它们的免疫反应性很弱，可能是从突触间间隙积累血清素的双极细胞的一个子集，并不是“真正的”5HT 神经元。 (C) 1997 ISDN。
Numerous neurotransmitters have been studied in detail in the developing retina. Almost all known neurotransmitters and neuromodulators were demonstrated in vertebrate retinas using formaldehyde-induced fluorescence, uptake autoradiography or immunohistochemistry procedures. Serotoninergic (5HT) amacrine neurons were described in the inner nuclear layer (INL) of the retina with their dendrites spreading within the inner plexiform layer (IPL). The present work describes the morphological pattern of development of serotoninergic amacrine neurons with a stratified dendritic branching pattern in the chick retina from embryonic day 12 to postnatal day 7. Serotoninergic-bipolar neurons are also described. 5HT-amacrine neurons have round or pear-shaped somata and primary dendritic trees oriented toward the IPL that runs through the INL, showing several varicosities. Secondary dendrites then go through the INL, without any collateral branch. At the outer and inner margin of the IPL the primary and secondary dendrites originate an outer and an inner serotoninergic network, respectively. When the primary dendritic tree reaches the IPL it deflects laterally in sublayer 1-the outer serotoninergic network. Tertiary branches then arise from the secondary dendrite and deflect in the innermost sublayer of the IPL-the inner serotoninergic network. The final pattern of branching of 5HT amacrine cells was present at embryonic day 14 and was completely developed at hatching. Serotoninergic (5HT) bipolar neurons were also present in the INL at hatching. They are weakly immunoreactive and are probably a subset of bipolar cells that accumulate serotonin from the intersynaptic cleft and are not ''true'' 5HT neurons. (C) 1997 ISDN.