INDUCTION OF DIFFERENTIATION IN HUMAN HL-60 CELLS BY 4-HYDROXYNONENAL, A PRODUCT OF LIPID-PEROXIDATION
INDUCTION OF DIFFERENTIATION IN HUMAN HL-60 CELLS BY 4-HYDROXYNONENAL, A PRODUCT OF LIPID-PEROXIDATION
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DOI：
10.1016/0014-4827(91)90416-r
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发表时间：
1991-12-01
影响因子：
3.7
通讯作者：
DIANZANI, MU
中科院分区：
文献类型：
--
作者：
BARRERA, G;DIMAURO, C;DIANZANI, MU
羟基壬烯醛（HNE）是细胞膜脂质过氧化反应产生的主要毒性物质。脂质过氧化水平及其产物浓度与细胞增殖速率呈负相关，与细胞分化水平直接相关。本文研究了HNE对HL-60人早幼粒细胞系增殖和分化的影响。以45分钟间隔用HNE（1 μM）重复处理，以使细胞在醛存在下维持7、2或9小时。HNE对细胞增殖和分化的影响与二甲基亚砜（DMSO）处理的细胞进行了比较。HNE引起细胞生长的强烈抑制而不影响细胞活力。此外，HL-60细胞获得的能力，产生化学发光后可溶性（佛波醇肉豆蔻酸酯乙酸酯）或corpuscolate（酵母聚糖）刺激。吞噬能力也通过计算吞噬调理酵母聚糖的细胞数量来计算。10或12次HNE处理后的值分别为43%和55%，DMSO处理的细胞为88%。髓过氧化物酶活性，治疗后5天，下降了85%，无论是HNE或DMSO处理的细胞，而酸性磷酸酶活性增加，相对于未处理的细胞。所获得的结果表明，HNE在接近正常组织中发现的浓度下可诱导HL-60细胞系的增殖抑制和分化诱导。
Hydroxynonenal (HNE) is the major diffusible toxic product generated by lipid peroxidation of cellular membranes. The level of lipid peroxidation and, consequently, the concentration of its products are inversely related to the rate of cell proliferation and directly related to the level of cell differentiation. In the present paper the effects of HNE on the proliferation and differentiation of the HL-60 human promyelocytic cell line have been investigated. Repeated treatment at 45-min intervals with HNE (1 μM) was performed to maintain the cells in the presence of the aldehyde for 7 1 2 or 9 h. The effect of HNE on cell proliferation and differentiation was compared with dimethyl sulfoxide (DMSO)-treated cells. HNE causes a strong inhibition of cell growth without affecting cell viability. Moreover, HL-60 cells acquire the capability to produce chemiluminescence after soluble (phorbol myristate acetate) or corpuscolate (zymosan) stimulation. The phagocytic ability has also been calculated by counting the number of cells that phagocytize opsonized zymosan. Values were 43 and 55% after 10 or 12 HNE treatments, respectively, and 88% in DMSO-treated cells. Myeloperoxidase activity, 5 days after treatment, decreased by 85% in either HNE-or DMSO-treated cells while acid phosphatase activity increased with respect to untreated cells. Results obtained indicate that HNE at concentrations close to those found in the normal tissues can induce inhibition of proliferation and induction of differentiation in the HL-60 cell line.