Mechanism for a Directional, Processive, and Reversible DNA Motor
Mechanism for a Directional, Processive, and Reversible DNA Motor
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DOI：
10.1002/smll.200900078
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发表时间：
2009-07-03
期刊：
影响因子：
13.3
通讯作者：
Turberfield, Andrew J.
中科院分区：
文献类型：
--
作者：
Bath, Jonathan;Green, Simon J.;Turberfield, Andrew J.
一个双足马达机制，协调化学和机械循环的两个相同的脚可以推广到获得能量的水解的DNA或RNA燃料被证明。可以在可重复使用的轨道上连续运行的电机需要单独的燃料、线性运动，并且可以通过使用电机架构耦合到ATP水解。燃料和发动机之间的相互作用可以通过在5英寸端部用FAM标记燃料和在3英寸端部用塔姆拉标记燃料来进行，从而减少荧光共振能量转移（FRET）。所推导的机制具有创建化学燃料分子马达所需的性质，该分子马达既具有方向性又具有进行性，并且这些马达的方向偏差可以通过改变燃料来逆转。
A bipedal motor mechanism that coordinates the chemical and mechanical cycles of two-identical feet can be generalized to obtain energy from hydrolysis of a DNA or RNA fuel was demonstrated. The motors that can operate continuously on a reusable track require a separate fuel, linear motion and can be coupled to ATP hydrolysis by using a motor architecture. The interaction between fuel and motor can be followed by labeling a fuel at the 5 inches end with FAM and at the 3 inches with TAMRA, thereby decreasing the fluorescence resonance energy transfer (FRET). The mechanism derived has the properties required to create a chemically fueled molecular motor that is both directional and processive, and the directional bias of these motors can be reversed by changing the fuel.