IMMORTALIZATION OF PRIMARY HUMAN EPITHELIAL-CELLS BY CLONED CERVICAL-CARCINOMA DNA CONTAINING HUMAN PAPILLOMAVIRUS TYPE-16 E6/E7 OPEN READING FRAMES
IMMORTALIZATION OF PRIMARY HUMAN EPITHELIAL-CELLS BY CLONED CERVICAL-CARCINOMA DNA CONTAINING HUMAN PAPILLOMAVIRUS TYPE-16 E6/E7 OPEN READING FRAMES
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DOI：
10.1099/0022-1317-70-5-1261
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发表时间：
1989-05-01
影响因子：
3.8
通讯作者：
CONE, R
中科院分区：
文献类型：
--
作者：
KAUR, P;MCDOUGALL, JK;CONE, R
用从宫颈癌克隆的DNA的亚基因组片段转染原代人上皮细胞，所述亚基因组片段含有来自人乳头瘤病毒16型原型的推定转化基因E6和E7。产生了几种永生化细胞系，所有细胞系均保留肿瘤DNA并表达病毒基因，表明这些基因中的一种或两种足以使原代人生殖器角质形成细胞永生化。
Primary human epithelial cells were transfected with a subgenomic fragment of DNA cloned from a cervical carcinoma, containing the putative transforming genes E6 and E7 from the human papillomavirus type 16 prototype. Several immortalized cell lines were generated, all retaining tumour DNA and expressing the viral genes, suggesting that either one or both of these genes is sufficient for the immortalization of primary human genital keratinocytes.