ADSORPTION OF VIRUSES TO CHARGE-MODIFIED SILICA
ADSORPTION OF VIRUSES TO CHARGE-MODIFIED SILICA
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DOI：
10.1128/aem.49.1.91-95.1985
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发表时间：
1985-01-01
影响因子：
4.4
通讯作者：
GOYAL, SM
中科院分区：
文献类型：
--
作者：
ZERDA, KS;GERBA, CP;GOYAL, SM
本研究的目的是提供对病毒吸附的更清晰的认识，特别关注病毒颗粒与吸附剂表面之间静电相互作用的作用。研究了脊髓灰质炎病毒1型、呼肠孤病毒1型和呼肠孤病毒3型以及噬菌体MS-2和T2对表面带正电荷或负电荷的硅胶胶体的吸附性能。将病毒与二氧化硅结合，在pH 4.0、6.4和8.5的0.1离子强度缓冲液中进行吸附实验。经特定吸附周期搅拌后的样品离心成颗粒状吸附剂颗粒加上吸附的病毒，上清液检测未吸附的病毒。在pH值低于等电点时，即在有利于病毒粒子表面带正电荷的条件下，所有病毒都只吸附在带负电荷的二氧化硅上。相反，当pH值高于等电点时，即病毒表面带负电荷时，所有病毒都只吸附在带正电的二氧化硅上。在接近等电状态下的病毒吸附在所有类型的二氧化硅上，尽管程度较低。
The purpose of this study was to provide a clearer understanding of virus adsorption, focusing specifically on the role of electrostatic interactions between virus particles and adsorbent surfaces. The adsorption of poliovirus 1, reovirus types 1 and 3, and coliphages MS-2 and T2 to colloidal silica synthetically modified to carry positive or negative surface charge was evaluated. Adsorption experiments were performed by combining virus and silica in 0.1-ionic-strength buffers of pH 4.0, 6.4 and 8.5. Samples agitated for specified adsorption periods were centrifuged to pellet adsorbent particles plus adsorbed virus, and the supernatants were assayed for unadsorbed virus. All viruses adsorbed exclusively to negatively charged silica at pH values below their isoelectric points, i.e., under conditions favoring a positive surface charge on the virions. Conversely, all viruses adsorbed exclusively to positively charged silica at pH values above their isoelectric points, i.e., where virus surface charge is negative. Viruses in near-isoelectric state adsorbed to all types of silica, albeit to a lesser degree.