The ultrastructure of chemolithoautotrophic Gallionella ferruginea and Thiobacillus ferrooxidans as revealed by chemical fixation and freeze-etching
The ultrastructure of chemolithoautotrophic Gallionella ferruginea and Thiobacillus ferrooxidans as revealed by chemical fixation and freeze-etching
复制标题
化学固定和冷冻蚀刻揭示的化学自养型铁红加仑菌和氧化亚铁硫杆菌的超微结构
DOI：
10.1007/bf00413137
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发表时间：
1989
影响因子：
2.8
通讯作者：
H. Hanert
中科院分区：
文献类型：
--
作者：
Susanne Lütters;H. Hanert
用巯基乙酸钠溶解化能自养铁细菌Gallionellaferrugulus的无菌培养物中的大量排泄物，可以研究纯细胞悬浮液中的Gallionellaces细胞的超微结构，而不丧失任何活力或受到致密的铁柄纤维的干扰，并与同样以亚铁为能源的化能自养生长的氧化亚铁硫杆菌进行比较。这两种生物体都是化学固定或冷冻蚀刻的。可以确定这些铁细菌之间的特殊结构差异。ferruginea具有胞质膜和可溶性核酮糖-1，5-二磷酸羧化酶，而T.铁氧化物含有羧化酶体，但无胞质膜;氧化亚铁只产生糖原。这两种生物体的细胞被膜层的冷冻断裂行为也彼此不同。而T. ferrooxidansexchloride具有特征性的双重分裂，暴露出外膜和细胞质膜的胞质破裂面和外质破裂面，G. ferrugine显示了层之间特别紧密的结合，导致仅细胞质膜内部断裂面的超分子组织的可视化。主要讨论的结果与这两种生物的极端不同的环境。
By using sodium thioglycolate to dissolve the high amount of excreted stalk material in axenic cultures of the chemolithoautotrophic iron bacteriumGallionella ferruginea, the ultrastructure ofGallionellacells from pure cell suspensions could be studied without any loss of viability or disturbance by dense ferric stalk fibers, and compared withThiobacillus ferrooxidans, also grown chemolithoautotrophically with ferrous iron as energy source. Both organisms were chemically fixed or freeze-etched. Particular structural differences between these iron-bacteria could be ascertained.G. ferrugineapossesses intracytoplasmic membranes and solubled-ribulose-1,5-bisphosphate-carboxylase, whereasT. ferrooxidanscontains carboxysomes but no intracytoplasmic membranes;Gallionellaforms poly-β-hydroxybutyrate and glycogen as storage material;T. ferrooxidansproduces only glycogen. Both organisms also differ from each other with respect to the freeze fracture behaviour of the cell envelope layers. Whereas the cells ofT. ferrooxidansexhibit a characteristic double cleavage, exposing the plasmic fracture face and exoplasmic fracture face of the outer membrane and cytoplasmic membrane, the exceptionally thin multilayered cell envelope ofG. ferruginearevealed a particularly intimate association between the layers, resulting in a visualisation of the supramolecular organisation of only the inner fracture face of the cytoplasmic membrane. The results are discussed predominantly in relation to the extremely distinct environments of both organisms.