Generation of Temperature Sensitive Mutations with Error-Prone PCR in a Gene Encoding a Component of the Spindle Pole Body in Fission Yeast
Generation of Temperature Sensitive Mutations with Error-Prone PCR in a Gene Encoding a Component of the Spindle Pole Body in Fission Yeast
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DOI：
10.1080/09168451.2019.1611414
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发表时间：
2019-04
期刊：
影响因子：
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通讯作者：
N. H. Tang;C. S. Fong;H. Masuda;I. Jourdain;M. Yukawa;T. Toda
中科院分区：
文献类型：
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作者：
N. H. Tang;C. S. Fong;H. Masuda;I. Jourdain;M. Yukawa;T. Toda
温度敏感突变体为研究必需基因的细胞功能提供了强有力的工具。我们在这里报告一个简单的程序，以产生TS突变，使用易错PCR在pcp1编码的纺锤杆体（SPB）组件裂殖酵母。这种操作并不局限于Pcp1的分析，并且可以适用于参与其他过程的任何必需基因。
Temperature-sensitive (ts) mutants provide powerful tools, thereby investigating cellular functions of essential genes. We report here a simple procedure to generate ts mutations using error-prone PCR in pcp1 that encodes a spindle pole body (SPB) component in Schizosaccharomyces pombe. This manipulation is not restricted to analysis of Pcp1, and can be suited to any essential genes involved in other processes.