A dielectrophoretic continuous flow sorter using integrated microelectrodes coupled to a channel constriction
A dielectrophoretic continuous flow sorter using integrated microelectrodes coupled to a channel constriction
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DOI：
10.1002/elps.201000675
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发表时间：
2011-06-01
期刊：
影响因子：
2.9
通讯作者：
Nicu, Liviu
中科院分区：
文献类型：
--
作者：
Salomon, Sven;Leichle, Thierry;Nicu, Liviu
在这篇文章中，我们提出了一种新的介电泳连续流分选机使用平面微电极耦合到一个通道收缩。这种设计能够在低电压下实现高颗粒分选效率，同时依赖于简单的制造和集成工艺。我们数值模拟了交流电动效应和流体行为来预测颗粒轨迹。模拟结果与实验数据一致：在100 μ m/s的流速下，10和5 μ m的聚苯乙烯珠被连续分选，误差< 2%。我们还能够在分选珠子时改变颗粒缓冲液。最后，为了证明我们的设备对细胞分选的兴趣，我们还根据它们不同的介电特性对死的和活的酵母细胞进行了分选。活细胞浓度相对于通过分选装置后的死细胞浓度富集4倍。
In this article, we propose a novel dielectrophoretic continuous flow sorter using planar micro electrodes coupled to a channel constriction. This design enables a high particle sorting efficiency at low voltages while relying on a simple fabrication and integration process. We have numerically simulated the AC electrokinetic effects and the fluid behavior to predict particle trajectories. Simulation results are in accordance with experimental data: 10 and 5 mu m polystyrene beads were continuously sorted with < 2% errors at flow speeds of 100 mu m/s. We were also able to change the particle buffer while sorting beads. Finally, to demonstrate the interest of our device for cell sorting, we also sorted dead and living yeast cells according to their different dielectric properties. Living cell concentration was enriched by a factor of 4 versus dead cell concentration after passing the sorting device.