Regulation of the growth of nonmuscle heart cells in culture.
Regulation of the growth of nonmuscle heart cells in culture.
复制标题
培养中非肌肉心脏细胞生长的调节。
DOI：
10.1007/bf02620924
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Daood,M
中科院分区：
文献类型：
--
作者：
Klein,I;Daood,M
新生大鼠心脏的原代培养物含有横纹肌（肌细胞）和非肌肉心脏细胞（NMHC）。尽管肌细胞在培养物中不分裂，但 NMHC 的数量确实会增加。 NMHC 的生长取决于培养基中血清浓度，范围为 1% 至 10%。与 10% 的生长相比，1% 血清中的细胞倍增时间更长，达到的最大密度低 70%。因此，支持正常肌细胞发育的 1% 血清是一种有用的培养基，由于它不能支持最佳的 NMHC 分裂，因此也可以维持肌肉心肌细胞的同质性。
Primary cultures of neonatal rat hearts contain both striated muscle (myocytes) as well as nonmuscle heart cells (NMHC). Although myocytes do not divide in culture, NMHC do increase in number. The growth of NMHC is dependent on the concentration of serum in the media over a range of 1 to 10%. When compared to growth in 10%, cells in 1% serum have a prolonged doubling time and reach a maximum density that is 70% less. Thus, 1% serum which supports normal myocyte development is a useful culture media to also maintain muscle heart cell homogeneity by its failure to support optimum NMHC division.