Further characterization of human eosinophil peroxidase.
Further characterization of human eosinophil peroxidase.
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人嗜酸性粒细胞过氧化物酶的进一步表征。
DOI：
10.1042/bj2290779
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
T. Christensen
中科院分区：
文献类型：
--
作者：
R. L. Olsen;K. Syse;C. Little;T. Christensen
在还原条件下通过凝胶过滤分离人嗜酸性粒细胞过氧化物酶的大亚基和小亚基（分别为Mr 50 000和10 500）。这些亚基关联性非常强，但没有明显通过二硫桥交联。在储存过程中，大的亚基往往会形成聚集体，需要还原才能解离它们。过甲酸处理的大亚基的氨基酸分析显示存在 19 个磺基丙氨酸残基。嗜酸性粒细胞过氧化物酶小亚基与髓过氧化物酶小亚基具有相同的Mr值。然而，尽管这些亚基具有非常相似的氨基酸组成，但在 CNBr 处理后它们表现出不同的肽断裂模式。嗜酸性粒细胞过氧化物酶的碳水化合物似乎仅与大亚基相关，并包含甘露糖（4.5％，w/w）和N-乙酰氨基葡萄糖（0.8％，w/w）。远-u.v.c.d。该酶的光谱表明存在相对较少的有序二级结构。
The large and the small subunits (Mr 50 000 and 10 500 respectively) of human eosinophil peroxidase were isolated by gel filtration under reducing conditions. The subunits were very strongly associated but not apparently cross-linked by disulphide bridges. During storage, the large subunit tended to form aggregates, which required reduction to dissociate them. Amino acid analysis of the performic acid-treated large subunit showed the presence of 19 cysteic acid residues. The small subunit of eosinophil peroxidase had the same Mr value as the small subunit of myeloperoxidase. However, although these subunits have very similar amino acid compositions, they showed different patterns of peptide fragmentation after CNBr treatment. The carbohydrate of eosinophil peroxidase seemed associated exclusively with the large subunit and comprised mannose (4.5%, w/w) and N-acetylglucosamine (0.8%, w/w). The far-u.v.c.d. spectrum of the enzyme indicated the presence of relatively little ordered secondary structure.