Identification and Validation of QTLs for Yield and Yield Components under Long-Term Salt Stress Using IR64 CSSLs in the Genetic Background of Koshihikari and Their Backcross Progenies
Identification and Validation of QTLs for Yield and Yield Components under Long-Term Salt Stress Using IR64 CSSLs in the Genetic Background of Koshihikari and Their Backcross Progenies
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DOI：
10.3390/agriculture11080777
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发表时间：
2021-08-01
影响因子：
3.6
通讯作者：
Hirai, Yoshihiko
中科院分区：
文献类型：
--
作者：
Nguyen Sao Mai;Dao Duy Hanh;Hirai, Yoshihiko
阐明耐盐性复杂的遗传基础和机制对培育耐盐品种具有重要意义。本研究以越光为遗传背景，对42个携带IR 64染色体片段的染色体片段代换系进行了盐胁迫评价。在胁迫条件下，SL 2007和SL 2038的植株干重和籽粒产量均高于越光。齐穗期叶片和全株Na+、Cl-积累量较低，这可能与其产量和产量构成因素较高有关。为了解其产量及产量构成因素的遗传控制，构建了SL 2007/越光F-2群体，并对其进行了数量性状基因座（QTL）分析。在第2染色体上检测到6个与产量及产量相关性状相关的QTL。使用来自SL 2007/越光F-5群体的近等基因系（NIL），qSTGY2.2被界定为2.5Mb区域，并且新qSTPN 2被界定为0.6Mb区域。我们还检测到一个新的QTL，qSTGF 2，籽粒灌浆，这被认为是一个重要的贡献者在盐胁迫下的粮食产量在这个CSSL。本研究结果为盐胁迫下水稻产量的形成机制提供了新的思路，也为水稻的克隆和育种提供了新的遗传资源。
Unraveling the complex genetic bases and mechanisms underlying salt tolerance is of great importance for developing salt-tolerant varieties. In this study, we evaluated 42 chromosome segment substitution lines (CSSLs) carrying chromosome segments from IR64 on the genetic background of Koshihikari under salt stress. Two CSSLs, SL2007 and SL2038, produced higher plant dry weight and grain yield than did Koshihikari under the stress condition. These CSSLs also showed lower Na+ and Cl- accumulation in the leaf and whole plant at the full heading stage, which might be related to the higher grain yield and yield components. To understand the genetic control of its grain yield and yield components, a SL2007/Koshihikari F-2 population was generated for quantitative trait locus (QTL) analysis. Six QTLs for grain yield and yield-related traits were detected on chromosome 2. Using near-isogenic lines (NILs) from a SL2007/Koshihikari F-5 population, qSTGY2.2 was delimited to a 2.5 Mb region and novel qSTPN2 was delimited to a 0.6 Mb region. We also detected a novel QTL, qSTGF2, for grain filling, which was considered an important contributor to grain yield under salt stress in this CSSL. Our results provide insights into mechanisms conferring grain yield under salinity stress and new genetic resources for cloning and breeding.