Universal primers for the PCR amplification of Dicot-infecting geminiviruses
Universal primers for the PCR amplification of Dicot-infecting geminiviruses
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DOI：
10.1007/bf02921559
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发表时间：
1994-01-01
影响因子：
2.6
通讯作者：
Markham, P. G.
中科院分区：
文献类型：
--
作者：
Briddon, R. W.;Markham, P. G.
双生病毒是一组环状单链DNA病毒，可导致热带和亚热带地区经济作物和自给自足作物的一些重大疾病(1)。最近，双生病毒引起的疾病在更温和的环境中的发病率一直在增加，包括欧洲和美国的南部地区(2，3)。感染双生病毒的双生病毒有单部分或双部分基因组(指定的基因组成分DNA A和DNA B)，并由叶蝉或粉虱传播(4)。已有报道用于扩增粉虱传播的双生病毒约一半的DNA A基因组成分的引物(5)。我们报道了一对简并引物的序列，该序列适用于感染双子叶植物的双生病毒近全长基因组(或DNA A基因组组分)的PCR介导的扩增。这些扩增产物(跨越基因组的17个碱基对以外的所有片段)不仅可用作诊断探针，还可用于在克隆病毒之前确定DNA序列和单切限制性内切酶位点。
Geminiviruses are a group of circular, single-stranded DNA viruses that cause a number of significant diseases of cash and subsistence crops in tropical and subtropical areas (1). Recently, the incidence of diseases caused by geminiviruses has been increasing in more temperate environments, including southern regions of Europe and the United States (2, 3). Dicotinfecting geminiviruses have either monopartite or bipartite (designated genomic components DNA A and DNA B) genomes and are transmitted by either leafhoppers or whiteflies (4).Primers for the amplification of approximately half the DNA A genomic components of whitefly-transmitted geminiviruses have been reported (5). We report the sequences of a pair of degenerate primers suitable for the PCR-mediated amplification of near full-length genomes (or DNA A genomic components) of geminiviruses that infect dicotyledonous plants. The products of these amplifications (spanning all but 17 bp of the genome) are useful not only as diagnostic probes but also for the determination of DNA sequence and single-cutting restriction endonuclease sites prior to cloning the viruses.