Vibrio natriegens: an ultrafast-growing marine bacterium as emerging synthetic biology chassis
Vibrio natriegens: an ultrafast-growing marine bacterium as emerging synthetic biology chassis
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DOI：
10.1111/1462-2920.15128
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发表时间：
2020-07-16
影响因子：
5.1
通讯作者：
Fritz, Georg
中科院分区：
文献类型：
--
作者：
Hoff, Josef;Daniel, Benjamin;Fritz, Georg
海洋细菌产钠弧菌是迄今为止已知的生长最快的非致病性细菌，作为大肠杆菌的替代底盘生物越来越受到关注。最近一波的合成生物学的努力集中在建立分子生物学工具，在这个迷人的有机体，现在使令人兴奋的应用-从加快我们的日常实验室程序，以增加生物技术的生产周期的步伐。natriegens，从最初的分离到最新的应用。我们讨论了它的自然生态位和与其他生物体的相互作用，揭示了它的一些非凡的特征，回顾了它的基因组组织，并深入了解了它的多样性代谢-进一步将这种生物体发展成为合成生物学底盘所需的关键生理学见解。通过对现有的遗传工具、方法和应用进行全面概述，我们强调了这种生物体目前的可能性，但也确定了一些可能推动未来研究的差距，希望能刺激V的增长。natriegens研究社区。
The marine bacteriumVibrio natriegensis the fastest-growing non-pathogenic bacterium known to date and is gaining more and more attention as an alternative chassis organism toEscherichia coli. A recent wave of synthetic biology efforts has focused on the establishment of molecular biology tools in this fascinating organism, now enabling exciting applications - from speeding up our everyday laboratory routines to increasing the pace of biotechnological production cycles.In this review, we seek to give a broad overview on the literature onV. natriegens, spanning all the way from its initial isolation to its latest applications. We discuss its natural ecological niche and interactions with other organisms, unveil some of its extraordinary traits, review its genomic organization and give insight into its diverse metabolism - key physiological insights required to further develop this organism into a synthetic biology chassis. By providing a comprehensive overview on the established genetic tools, methods and applications we highlight the current possibilities of this organism, but also identify some of the gaps that could drive future lines of research, hopefully stimulating the growth of theV. natriegensresearch community.