Effect of different types of muscle activity on the gene and protein expression of ALDH family members in C57BL/6J mouse skeletal muscle
Effect of different types of muscle activity on the gene and protein expression of ALDH family members in C57BL/6J mouse skeletal muscle
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不同类型肌肉活动对C57BL/6J小鼠骨骼肌ALDH家族成员基因及蛋白表达的影响
DOI：
10.1139/apnm-2022-0005
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Nakazato Koichi
中科院分区：
文献类型：
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作者：
Jee Eunbin;Tamura Yuki;Kouzaki Karina;Kotani Takaya;Nakazato Koichi
乙醛脱氢酶（ALDH）是一种解毒醛的酶，主要参与酒精代谢。最近，我们发现 ALDH 在骨骼肌稳态中也发挥着重要作用。为了更好地了解ALDH在骨骼肌中的作用，有必要明确ALDH的适应性。在这项研究中，我们研究了耐力训练、增效剂消融 (SA) 引起的代偿性肥大以及去神经诱导的萎缩对骨骼肌中选定 ALDH 亚型的基因表达和蛋白质水平的影响。十周龄的 C57BL/6J 小鼠接受每次干预，并收集跖肌。 SA和去神经支配使Aldh1a1基因表达水平降低，但ALDH1A1蛋白水平不受影响。长期耐力训练、SA 和去神经干预后，ALDH1B1 的蛋白质水平增加。然而，仅在 SA 后才观察到 Aldh1b1 基因表达的增加。 SA后Aldh2基因表达量下降，但ALDH2蛋白水平保持不变。去神经支配增加了Aldh2基因和ALDH2蛋白水平。总而言之，每种 ALDH 异构体在不同条件下都会在骨骼肌中经历独特的定量适应。
Aldehyde dehydrogenase (ALDH) is an enzyme that detoxifies aldehydes and is primarily involved in alcohol metabolism. Recently, we have shown that ALDH also plays an important role in skeletal muscle homeostasis. To better understand the role of ALDH in skeletal muscle, it is necessary to clarify the adaptability of ALDH. In this study, we examined the effects of endurance training, compensatory hypertrophy by synergist ablation (SA), and denervation-induced atrophy on gene expression and protein levels of selected ALDH isoforms in skeletal muscle. Ten-week-old C57BL/6J mice were subjected to each intervention, and the plantaris muscle was collected. Gene expression levels ofAldh1a1were decreased by SA and denervation, but ALDH1A1 protein levels were not affected. Protein levels of ALDH1B1 increased after chronic endurance training, SA, and denervation interventions. However, the increase inAldh1b1gene expression was observed only after SA. The gene expression ofAldh2was decreased after SA, but ALDH2 protein levels remained unchanged. Denervation increased both theAldh2gene and ALDH2 protein levels. Taken together, each isoform of ALDH undergoes unique quantitative adaptations in skeletal muscle under different conditions.