Associations between Variation in X Chromosome Male Reproductive Genes and Sperm Competitive Ability in Drosophila melanogaster.
Associations between Variation in X Chromosome Male Reproductive Genes and Sperm Competitive Ability in Drosophila melanogaster.
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DOI：
10.4061/2011/214280
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发表时间：
2011-01-01
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影响因子：
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通讯作者：
Clark, Andrew G
中科院分区：
文献类型：
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作者：
Greenspan, Leah;Clark, Andrew G
长期以来，生殖成功的差异一直被认为部分是由编码精液蛋白的基因介导的。在这里，我们探索X连锁多态对男性生殖表型的影响，X连锁多态是一种依赖于编码精液蛋白的基因的染色体。使用57个X染色体替代系，测试了两种情况下的精子竞争：第一次交配的是来自野生系的雄性(“防御性”杂交)，第二次交配的是第二次交配的雄性(“攻击性”杂交)。对各雄株的父本比例、繁殖力、再生率和不育性进行评分，并对品系间变异的显著性进行检验。根据先前的研究选择了11个候选基因，并对所有57个品系的部分基因进行了测序。使用线性模型对131个多态与生殖表型的相关性进行了检验。在4个基因中的9个多态被发现显示出显著的相关性(在5%的FDR)。总体而言，X染色体似乎在精子竞争中蕴含着丰富的变异，特别是考虑到精液蛋白基因的缺乏。这表明，大部分男性生殖变异存在于编码精液蛋白的基因之外。
Variation in reproductive success has long been thought to be mediated in part by genes encoding seminal proteins. Here we explore the effect on male reproductive phenotypes of X-linked polymorphisms, a chromosome that is depauperate in genes encoding seminal proteins. Using 57 X chromosome substitution lines, sperm competition was tested both when the males from the wild-extracted line were the first to mate ("defense" crosses), followed by a tester male, and when extracted-line males were the second to mate, after a tester male ("offfense" crosses). We scored the proportion of progeny sired by each male, the fecundity, the remating rate and refractoriness to remating, and tested the significance of variation among lines. Eleven candidate genes were chosen based on previous studies, and portions of these genes were sequenced in all 57 lines. A total of 131 polymorphisms were tested for associations with the reproductive phenotypes using linear models. Nine polymorphisms in 4 genes were found to show significant associations (at a 5% FDR). Overall, it appears that the X chromosomes harbor abundant variation in sperm competition, especially considering the paucity of seminal protein genes. This suggests that much of the male reproductive variation lies outside of genes that encode seminal proteins.