Reagentless colorimetric biosensing platform based on nanoceria within an agarose gel matrix.
Reagentless colorimetric biosensing platform based on nanoceria within an agarose gel matrix.
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DOI：
10.1016/j.bios.2016.08.113
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发表时间：
2017-07
影响因子：
12.6
通讯作者：
Do Hyun Kim;Jaehyun Hur;H. Park;Moon Il Kim
中科院分区：
文献类型：
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作者：
Do Hyun Kim;Jaehyun Hur;H. Park;Moon Il Kim
由氧化铈纳米颗粒（nanoceria）和氧化酶共包埋在琼脂糖凝胶组成的复合材料已被开发用于无试剂比色检测生物学上重要的目标分子。固定在琼脂糖基质中的氧化酶促进靶分子的氧化以产生H2 O2，随后诱导纳米氧化铈的物理化学性质发生变化，表现出从白色/浅黄色到深黄色/浅橙色的颜色变化，而不需要任何额外的比色底物。通过利用包埋在琼脂糖凝胶内的纳米二氧化铈的独特变色性质，在0.05至2 mM的宽线性范围内非常特异性地检测目标葡萄糖分子，这适合于测量血清葡萄糖水平，在室温下两周内具有优异的操作稳定性。当用于检测存在于真实的人血清样品中的葡萄糖时，该生物传感器也表现出高度的精确度和再现性。我们期望这种新的基于纳米二氧化硅的生物传感形式可以很容易地扩展到其他氧化酶，以方便检测各种临床重要的靶分子。
A composite consisting of cerium oxide nanoparticles (nanoceria) and an oxidative enzyme co-entrapped in an agarose gel has been developed for the reagent-free colorimetric detection of biologically important target molecules. The oxidase, immobilized in the agarose matrix, promotes the oxidation of target molecules to generate H2O2that subsequently induces changes in the physicochemical properties of nanoceria exhibiting a color change from white/light-yellow into intense-yellow/light-orange without any requirement for additional colorimetric substrates. By utilizing the unique color-changing property of nanoceria entrapped within the agarose gel, target glucose molecules were very specifically detected over a wide linear range from 0.05 to 2 mM, which is suitable to measure the serum glucose level, with excellent operational stability over two weeks at room temperature. The biosensor also exhibited a high degree of precision and reproducibility when employed to detect glucose present in real human serum samples. We expect that this novel nanoceria-based biosensing format could be readily extended to other oxidative enzymes for the convenient detection of various clinically important target molecules.