Effects of dietary sodium acetate on food intake, weight gain, intestinal digestive enzyme activities, energy metabolism and gut microbiota in cultured fish: Zebrafish as a model
Effects of dietary sodium acetate on food intake, weight gain, intestinal digestive enzyme activities, energy metabolism and gut microbiota in cultured fish: Zebrafish as a model
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膳食醋酸钠对养殖鱼食物摄入、体重增加、肠道消化酶活性、能量代谢和肠道微生物群的影响：以斑马鱼为模型
DOI：
10.1016/j.aquaculture.2020.735188
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发表时间：
2020
期刊：
影响因子：
4.5
通讯作者：
Zhou Zhigang
中科院分区：
文献类型：
--
作者：
Zhang Hongling;Ding Qianwen;Wang Anran;Liu Yu;Teame Tsegay;Ran Chao;Yang Yalin;He Suxu;Zhou Wenhao;Olsen Rolf Erik;Zhang Zhen;Zhou Zhigang
醋酸盐是鱼类胃肠道中含量最丰富的短链脂肪酸。研究饲料中添加乙酸钠（NaAc）对斑马鱼摄食量、增重、肠道消化酶活性、能量代谢和肠道菌群的影响。本研究以0.15%NaAc饲料喂饲1月龄斑马鱼4周，在饱食状态下进行摄食试验。在试验结束时，与对照组相比，添加0.15%NaAc的饲料显著提高了肠道乙酸水平（p<0.05），并伴随着较高的增重和日摄食率（p<0.05）。饲料中添加0.15%NaAc显著提高了鱼体脂肪质量、能量增益和能量转化效率以及肠道消化酶（淀粉酶）活性（p<0.05）。饲喂接触0.15%NaAc饲料的鱼脑中摄食相关基因（ghre、npy和npy 7R）的表达高于对照组。脑室内注射NaAc后，斑马鱼摄食相关基因的表达也明显增加。NaAc对斑马鱼脑内食欲相关基因表达的影响可被阿托品阻断，表明醋酸盐通过副交感神经系统直接参与食欲控制。此外，在饲料中添加NaAc的鱼肠道中邻单胞菌属的相对丰度显著降低（p<0.05）。这些数据表明，醋酸钠具有潜在的价值，作为鱼类饲料添加剂在水产养殖。
Acetate is the most abundant short-chain fatty acids (SCFAs) in the gastrointestinal tract of fish. The aim of this study was to investigate the effects of dietary sodium acetate (NaAc) on food intake, weight gain, intestinal digestive enzyme activities, energy metabolism and gut microbiota of zebrafish (Danio rerio). In this study, 1-month-old zebrafish were fed with 0.15% NaAc diet for 4 weeks under satiation feeding. At the end of feeding trial, the level of intestinal acetate in the fish fed with 0.15% NaAc containing diet were significantly increased (p< .05), which was accompanied by a higher weight gain and daily feeding rate compared with the control group (p< .05). Dietary supplementation of 0.15% NaAc significantly promoted the body fat mass, energy gain and energy conversion efficiency, as well as intestinal digestive enzyme (amylase) activity in fish (p< .05). The expression of food intake-related genes (ghre,npyandnpy7R) in the brain of fish fed on the diet contacting 0.15% NaAc was higher than that of the control group. Consistently, intracerebroventricular injection of NaAc in the brain of zebrafish also increased the expression of genes related to food intake. The effects of NaAc on orexigenic genes expression in the brain of zebrafish could be prevented by co-injection of atropine, suggesting that acetate played a direct role in controlling appetite through the parasympathetic nerve system. Moreover, there was significantly decrease (p< .05) in the relative abundance ofPlesiomonasgenus in the fish gut fed on the diet supplemented with NaAc. These data suggest that NaAc has a potential value as fish feed additive in aquaculture.