In vitro astrocyte and cerebral endothelial cell response to electrospun poly(ε-caprolactone) mats of different architecture
In vitro astrocyte and cerebral endothelial cell response to electrospun poly(ε-caprolactone) mats of different architecture
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DOI：
10.1007/s10856-009-3944-5
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发表时间：
2010-04-01
影响因子：
3.7
通讯作者：
Folin, Marcella
中科院分区：
文献类型：
--
作者：
Baiguera, Silvia;Del Gaudio, Costantino;Folin, Marcella
这项工作的重点是评价潜在的使用静电纺聚（ε-己内酯）（PCL）微米和/或亚微米纤维膜的大鼠海马星形胶质细胞（HA）和大鼠海马微血管内皮细胞（CEC）的文化。两种垫均支持细胞粘附、增殖、细胞表型和铺展。微纤维垫允许细胞浸润，而HA和CEC都不能在亚微米纤维垫内迁移，留下脱细胞的内部区域。这一发现与静电纺丝PCL微纤维垫内存在较大空隙相关，表明应准确选择形态以实现细胞环境模拟垫。根据我们的研究结果，适当的纤维结构可以被视为一个关键的问题，要考虑，以处理适合特定的体外应用的聚合物垫。
This work focuses on the evaluation of the potential use of electrospun poly(epsilon-caprolactone) (PCL) micrometric and/or sub-micrometric fibrous membranes for rat hippocampal astrocyte (HA) and rat cerebro-microvascular endothelial cell (CEC) cultures. Both mats supported cell adhesion, proliferation, cellular phenotype and spreading. Microfibrous mats allowed cellular infiltration, while both HAs and CECs were unable to migrate within the sub-micrometric fibrous mat, leaving an acellularized inner region. This finding was correlated to the presence of larger voids within electrospun PCL microfibrous mats, suggesting that the morphology should be accurately selected for the realization of a cell environment-mimicking mat. Based on our results, the proper fiber architecture can be regarded as a crucial issue to be considered in order to deal with suitable polymeric mats tailored for specific in vitro application.