Plasmatic MicroRNA Signatures in Elderly People with Stable and Unstable Angina
Plasmatic MicroRNA Signatures in Elderly People with Stable and Unstable Angina
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患有稳定型和不稳定型心绞痛的老年人的血浆 MicroRNA 特征
DOI：
10.1536/ihj.17-063
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发表时间：
2018-01-01
影响因子：
1.5
通讯作者：
Chen, Hong
中科院分区：
文献类型：
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作者：
Cui, Yuxia;Song, Junxian;Chen, Hong
本研究旨在探讨老年不稳定型心绞痛（UA）和稳定型心绞痛（SA）患者血浆中miRNA的差异，寻找老年UA的有效标志物。采用微阵列技术检测10例老年UA患者和10例老年SA患者血浆中miRNA的表达水平，并采用Real-time PCR技术进行验证。在验证期间，Mir-1202、mir-1207- 5 p和mir-1225- 5 p显示统计学显著的下调（P < 0.05），而mir-3162- 3 p显示上调（P < 0.05）。在所有单个miRNA中，miR-3162- 3 p在老年UA患者的诊断中显示出最高的区分能力（AUC：0.79，95%CI：0.675-0.905）。一组三种miRNAs的区分能力（mir-3162- 3 p/mir-1225- 5 p/mir-1207- 5 p）的AUC最高，为0.91（95%CI：0.84-0.98），随后是mir-3162- 3 p/mir-1225- 5 p（AUC：0.833，95%CI：0.732-0.934）和mir-3162- 3 p/mir-1207- 5 p（AUC：0.817，95%CI：0.712-0.922）。结论：多miRNA联合检测对老年UA患者的诊断具有较高的价值。
We aimed to investigate the distinctive miRNA profiles in the plasma of elderly patients with unstable angina (UA) and stable angina (SA), and to find more effective markers of UA in elderly people. We compared miRNA expression levels in plasma samples from 10 elderly patients with UA and 10 elderly patients with SA by using microarray-based miRNA chip, and then performed validation with Real-time PCR. Mir-1202, mir-1207-5p, and mir-1225-5p showed a statistically significant down-regulation (P < 0.05), while mir-3162-3p showed an up-regulation (P < 0.05) during validation. Among all single miRNAs, miR-3162-3p showed the highest discriminatory power in the diagnosis of elderly patients with UA (AUC: 0.79, 95% CI: 0.675-0.905). The discriminatory power of a panel of three miRNAs (mir-3162-3p/mir-1225-5p/mir-1207-5p) was highest with an AUC of 0.91 (95% CI: 0.84-0.98), followed by mir-3162-3p/mir-1225-5p (AUC: 0.833, 95% CI: 0.732-0.934) and mir-3162-3p/mir-1207-5p (AUC: 0.817, 95% CI: 0.712-0.922). In conclusion, multi-miRNA panel could provide higher diagnostic value for the diagnosis of elderly patients with UA.