Taking Orders from Light: Photo-Switchable Working/Inactive Smart Surfaces for Protein and Cell Adhesion.
Taking Orders from Light: Photo-Switchable Working/Inactive Smart Surfaces for Protein and Cell Adhesion.
复制标题
DOI：
10.1021/acsami.6b15599
复制
发表时间：
2017-03
影响因子：
9.5
通讯作者：
Junji Zhang;Wenjing Ma;Xiao‐Peng He;H. Tian
中科院分区：
文献类型：
--
作者：
Junji Zhang;Wenjing Ma;Xiao‐Peng He;H. Tian
光响应智能表面在光电子学和传感器件中具有广泛的应用前景。使用光作为命令信号提供了具有高时间和空间分辨率的远程和非侵入性控制的优点。用目标生物大分子（如肽、DNA和包括叶酸衍生物和糖的小分子）修饰光开关最近已成为使智能表面具有改进的检测效率和特异性的流行策略。在此，我们报告的光开关自组装单分子膜（SAMs）的基础上糖（半乳糖/甘露糖）修饰的偶氮苯衍生物的建设，并确定其光开关，选择性蛋白质/细胞粘附性能通过电化学。在交替的UV/维斯照射下，实现了对选择性凝集素（识别糖的蛋白质）和高度表达糖受体的细胞的可相互转换的高/低识别和结合亲和力。此外，对选择性蛋白质和细胞具有低结合亲和力的顺式-SAM也对非选择性蛋白质和细胞样品表现出最小的响应，这提供了在用于实际应用之前避免不必要的污染和探针消耗的可能性。此外，所使用的电化学技术促进了基于智能表面的便携式设备的开发，用于按需疾病诊断。
Photoresponsive smart surfaces are promising candidates for a variety of applications in optoelectronics and sensing devices. The use of light as an order signal provides advantages of remote and noninvasive control with high temporal and spatial resolutions. Modification of the photoswitches with target biomacromolecules, such as peptides, DNA, and small molecules including folic acid derivatives and sugars, has recently become a popular strategy to empower the smart surfaces with an improved detection efficiency and specificity. Herein, we report the construction of photoswitchable self-assembled monolayers (SAMs) based on sugar (galactose/mannose)-decorated azobenzene derivatives and determine their photoswitchable, selective protein/cell adhesion performances via electrochemistry. Under alternate UV/vis irradiation, interconvertible high/low recognition and binding affinity toward selective lectins (proteins that recognize sugars) and cells that highly express sugar receptors are achieved. Furthermore, the cis-SAMs with a low binding affinity toward selective proteins and cells also exhibit minimal response toward unselective protein and cell samples, which offers the possibility in avoiding unwanted contamination and consumption of probes prior to functioning for practical applications. Besides, the electrochemical technique used facilitates the development of portable devices based on the smart surfaces for on-demand disease diagnosis.