The geophagous earthworm Metaphire guillelmi effects on rhizosphere microbial community structure and functioning vary with plant species
The geophagous earthworm Metaphire guillelmi effects on rhizosphere microbial community structure and functioning vary with plant species
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地食性蚯蚓 Metaphire guillelmi 对根际微生物群落结构和功能的影响因植物种类而异
DOI：
10.1016/j.geoderma.2020.114647
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发表时间：
2020
期刊：
影响因子：
6.1
通讯作者：
Liu Manqiang
中科院分区：
文献类型：
--
作者：
Zheng Yong;Chen Xiaoyun;Gong Xin;Bonkowski Michael;Wang Shuai;Griffiths Bryan;Hu Feng;Liu Manqiang
植物资源的数量和质量是调节土壤微生物群落的主要因素，然而，土壤动物对土壤食物网的自上而下的控制仍未得到充分的探索。为了整合自下而上和自上而下调节土壤微生物群落的力量，我们评估了6种植物根际微生物群落结构(由多个PLFA生物标志物指示)和功能(由4种不同的酶活性指示)，在有和没有7种成虫存在和不存在的情况下。6种植物，2种豆科植物(白三叶和紫花苜蓿)，2种牧草(多年生黑麦草和多年生黑麦草)和2种粮食作物(普通小麦和玉米)，根据资源需求和养分获取策略而不同。我们发现，蚯蚓导致了所有研究植物物种根际微生物群落结构的相似性增加。例如，在蚯蚓存在的情况下，六种植物的真菌：细菌和革兰氏阳性：革兰氏阴性比率的可变性降低。此外，蚯蚓普遍支持酶的产生，以获取碳超过氮和磷，并加强对微生物生物量中N的固定化汇，这表明蚯蚓更有利于微生物获取能量，因为植物根部产生的酶主要针对营养。综上所述，我们的结果揭示了蚯蚓对微生物群落结构的上下文依赖性的影响，以及蚯蚓在不同植物物种之间转移酶产生的一般模式。
The quantity and quality of plant-derived resources are the primary factors regulating soil microbial communities, however, the top-down control of soil fauna on the soil food web is still underexplored. To integrate bottom-up and top-down forces in mediating soil microbial communities, we evaluated six plant species rhizosphere microbial community structure (indicated by multiple PLFA biomarkers) and functioning (indicated by four different enzyme activities) in the presence and absence of seven adultMetaphire guillelmiearthworms. Six plant species with two legume species (Trifolium repensandMedicago sativa), two grass species (Arundo donaxandLolium perenne) and two grain crops (Triticum aestivumandZea mays) were used varying with resource demand and nutrient acquisition strategies. We found earthworms led to increasing in similarity of rhizosphere microbial community structure across all studied plant species. For example, the variability of the fungi: bacteria and gram-positive: gram-negative ratios across six plant species was reduced in the presence of earthworms. Furthermore, earthworms generally supported enzyme production in acquiring carbon over nitrogen and phosphorus, and enhanced immobilization sink for N in microbial biomass, suggesting earthworms are more beneficial for microbes in acquiring energy as plant roots-produced enzyme mostly targeted on nutrients. In conclusion, our results revealed a context-dependent earthworm effects on microbial community structure and a general pattern of earthworms in shifting enzyme production across different plant species.