Difference in coughing and other responses to intrabronchial infection with Bordetella pertussis among strains of rats
Difference in coughing and other responses to intrabronchial infection with Bordetella pertussis among strains of rats
复制标题
DOI：
10.1128/iai.65.11.4711-4717.1997
复制
发表时间：
1997-11-01
影响因子：
3.1
通讯作者：
Wardlaw, AC
中科院分区：
文献类型：
--
作者：
Hall, E;Parton, R;Wardlaw, AC
用琼脂糖珠包裹大约10(8)CFU的百日咳杆菌18-323感染4株大鼠，8天后，用声控录音机监测，Sprague-Dawley大鼠咳嗽发作的发生率最高；褐挪威鼠、刘易斯鼠和帽状李斯特鼠咳嗽频率明显较低。在所有四种大鼠品系中都出现了明显的白细胞增多，其计数高达正常水平的四倍，并且正常体重增长迟缓。咳嗽和白细胞增多在4周龄感染的动物比在6周龄感染的动物更大。感染后第8天，4株大鼠血清总免疫球蛋白E （IgE）升高9 ~ 244倍，6周后恢复到接近感染前水平。Sprague-Dawley和Lewis大鼠血清中总IgE基础水平最低，但其升高程度最大。4株大鼠感染后6周血清中均有对百日咳全细胞超声抗体和丝状血凝素抗体的IgG。然而，不同的菌株在百日咳毒素IgG的产生上存在差异，其中Sprague-Dawley大鼠滴度最高，而hood Lister和Lewis大鼠无应答，这些研究强调了大鼠菌株作为百日咳大鼠模型变量的重要性，并验证了以往研究中Sprague-Dawley大鼠的选择。
Four strains of rats were each infected intrabronchially with approximately 10(8) CFU of Bordetella pertussis 18-323 encased in fine agarose beads, After 8 days, Sprague-Dawley rats developed the highest incidence of coughing paroxysms, as monitored with voice-activated tape recorders; Brown Norway, Lewis, and Hooded Lister rats coughed significantly less frequently. Marked leukocytosis, with counts up to four times the normal levels, and retardation of normal weight gain occurred in all four rat strains. Both coughing and leukocytosis were greater in animals that were infected at 4 weeks of age than in those infected at 6 weeks of age. Total serum immunoglobulin E (IgE) rose in all four rat strains 9- to 244-fold by day 8 after infection and returned to near preinfection levels at 6 weeks. Sprague-Dawley and Lewis rats, which had the lowest basal levels of total IgE in serum, showed the greatest degrees of elevation. All four rat strains had IgG to B. pertussis whole-cell sonicate and to filamentous hemagglutinin in 6-week-postinfection sera. However, the strains differed in production of IgG to pertussis toxin, with Sprague-Dawley rats having the highest titers and Hooded Lister and Lewis rats being nonresponders, These studies highlight the importance of rat strain as a variable in the coughing-rat model of pertussis and validate the choice of the Sprague-Dawley rats in previous studies.