DETECTION, ISOLATION, AND CONTINUOUS PRODUCTION OF CYTOPATHIC RETROVIRUSES (HTLV-III) FROM PATIENTS WITH AIDS AND PRE-AIDS
DETECTION, ISOLATION, AND CONTINUOUS PRODUCTION OF CYTOPATHIC RETROVIRUSES (HTLV-III) FROM PATIENTS WITH AIDS AND PRE-AIDS
复制标题
DOI：
10.1126/science.6200935
复制
发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
GALLO, RC
中科院分区：
文献类型：
--
作者：
POPOVIC, M;SARNGADHARAN, MG;GALLO, RC
开发了一种细胞系统，用于可重复检测获得性免疫缺陷综合征（AIDS）患者或具有AIDS前（AIDS前）体征或症状的患者中的人嗜T淋巴细胞逆转录病毒（HTLV [人T细胞白血病-淋巴瘤病毒]家族）。这些细胞是来自允许的人肿瘤性T细胞系的特异性克隆。一些克隆在感染这些病毒的致细胞病变（HTLV-III）变体后永久生长并持续产生大量病毒。HTLV-III在该系统中的一种细胞病变效应是在受感染的T细胞群的巨细胞中以特征性环状形成的多个核的排列。这些结构可用作检测临床标本中HTLV-III的指示剂。该系统为常规检测艾滋病患者或艾滋病前期患者和健康携带者中的HTLV-III和HTLV的相关细胞病变变体开辟了道路，并为详细的分子和免疫学分析提供了大量病毒。
A cell system was developed for the reproducible detection of human T-lymphotropic retroviruses (HTLV [human T cell leukemia-lymphoma virus] family) from patients with the acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that frequently precede AIDS (pre-AIDS). The cells were specific clones from a permissive human neoplastic T-cell line. Some of the clones permanently grew and continuously produced large amounts of virus after infection with cytopathic (HTLV-III) variants of these viruses. One cytopathic effect of HTLV-III in this system was the arrangement of multiple nuclei in a characteristic ring formation in giant cells of the infected T-cell population. These structures can be used as an indicator to detect HTLV-III in clinical specimens. This system opens the way to the routine detection of HTLV-III and related cytopathic variants of HTLV in patients with AIDS or pre-AIDS and in healthy carriers, and it provides large amounts of virus for detailed molecular and immunological analyses.