Mobility and cycling of synaptic protein-containing vesicles in axonal growth cone filopodia
Mobility and cycling of synaptic protein-containing vesicles in axonal growth cone filopodia
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DOI：
10.1038/nn1149
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发表时间：
2003-12-01
影响因子：
25
通讯作者：
McAllister, AK
中科院分区：
文献类型：
--
作者：
Sabo, SL;McAllister, AK
生长锥内泡状体动态的空间分布和协调知之甚少。长期以来，人们一直认为膜性细胞器集中在生长锥的中心区域，并被排除在丝状伪足之外;这一观点极大地塑造了轴突生长和突触前末端形成的细胞机制的概念模型。为了开始测试这些模型，我们研究了活的大鼠皮层神经元轴突生长锥内的膜动力学。我们表明，生长锥丝状伪足包含的囊泡，运输突触囊泡蛋白双向沿着丝状伪足和融合的丝状伪足表面在响应局灶性刺激，允许局部分泌囊泡内容物和快速变化的质膜组成的个别丝状伪足。我们的研究结果提出了一个新的模型，其中生长锥丝状伪足积极参与发射和响应与轴突生长和早期突触形成相关的局部信号。
The spatial distribution and coordination of vesicular dynamics within growth cones are poorly understood. It has long been thought that membranous organelles are concentrated in the central regions of growth cones and excluded from filopodia; this view has dramatically shaped conceptual models of the cellular mechanisms of axonal growth and presynaptic terminal formation. To begin to test these models, we studied membrane dynamics within axonal growth cones of living rat cortical neurons. We demonstrate that growth cone filopodia contain vesicles that transport synaptic vesicle proteins bidirectionally along filopodia and fuse with the filopodial surface in response to focal stimulation, allowing for both local secretion of vesicular contents and rapid changes in the plasma membrane composition of individual filopodia. Our results suggest a new model in which growth cone filopodia are actively involved in both emitting and responding to local signals related to axon growth and early synapse formation.