The male pseudohermaphrodite XX polled goat is Zfy and Sry negative.
The male pseudohermaphrodite XX polled goat is Zfy and Sry negative.
复制标题
雄性假雌雄同体 XX 无角山羊 Zfy 和 Sry 呈阴性。
作者：
W. Just;Carlos Cabral de Almeida;Beatriz Goldshmidt;Walther Vogel
山羊的无角突变是作为常染色体显性遗传的。具有 60,XX 核型的纯合子发育成双性体。我们通过 Southern blotting 和 PCR 分析，寻找雄性假雌雄同体无角山羊中是否存在 Y 染色体序列（Zfy 和 Sry）。分子分析显示，这只XX山羊没有Sry序列。使用低严格度的人类 SRY 和 ZFY 探针，它与正常 XX 雌性山羊表现出相同的杂交模式，而 XY 雄性山羊则显示出显着的 Sry 和 Zfy 信号。使用山羊 Sry 引物进行 PCR 分析获得了相同的结果。讨论了常染色体隐性基因在睾丸和男性的诱导中的可能作用。
The Polled mutation of the goat is inherited as an autosomal dominant trait. Homozygotes with a 60,XX karyotype develop into intersexes. We looked for the presence of Y-chromosomal sequences (Zfy and Sry) in a male pseudohermaphrodite polled goat by Southern blotting and PCR analysis. Molecular analysis revealed that this XX goat had no Sry sequence. It showed identical hybridization patterns, using the human SRY and ZFY probes at low stringency, with the normal XX female goat, whereas an XY male revealed prominent Sry and Zfy signals. Identical results were obtained from PCR analysis with the caprine Sry primers. The possible role of an autosomal recessive gene in the induction of testes and maleness is discussed.
Xp 的部分重复：病例报告和对先前报告病例的回顾。
DOI：
10.1002/ajmg.1320400306
发表时间：
1991
期刊：
American journal of medical genetics
影响因子：
--
作者：
Wyandt,HE;Bugeau-Michaud,L;Skare,JC;Milunsky,A
通讯作者：
Milunsky,A