Anti-Inflammatory Effects of a Methanol Extract from the Marine Sponge Geodia cydonium on the Human Breast Cancer MCF-7 Cell Line.
Anti-Inflammatory Effects of a Methanol Extract from the Marine Sponge Geodia cydonium on the Human Breast Cancer MCF-7 Cell Line.
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DOI：
10.1155/2015/204975
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发表时间：
2015
影响因子：
4.6
通讯作者：
Costantini M
中科院分区：
文献类型：
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作者：
Costantini S;Romano G;Rusolo F;Capone F;Guerriero E;Colonna G;Ianora A;Ciliberto G;Costantini M
许多研究小组正在努力寻找新的可能的抗炎分子，海洋海绵代表了具有药理应用的生物活性化合物的丰富来源。在本研究中，我们测试了不同浓度的海绵甲醇提取物对正常人乳腺上皮细胞（MCF-10A）和人乳腺癌细胞（MCF-7）的作用。我们的研究结果表明，这种提取物对两种细胞系都没有细胞毒性作用，而它只在MCF-7细胞中以剂量依赖的方式诱导VEGF和五种促炎细胞因子（CCL2, CXCL8, CXCL10， IFN-γ和TNF-α）的水平降低，从而表明抗炎作用。此外，相互作用分析表明，所有六种细胞因子都参与一个网络，并与一些HUB节点（如NF-kB亚基和ESR1（雌激素受体1））相连。我们还通过RT-qPCR实验报道，提取物处理后，仅在MCF-7细胞中NFKB1和c-Rel两个亚基的表达减少，证实NF-kB失活。这些数据突出了cydonium在未来针对重大疾病（如乳腺癌）的药物发现方面的潜力。
Many research groups are working to find new possible anti-inflammatory molecules, and marine sponges represent a rich source of biologically active compounds with pharmacological applications. In the present study, we tested different concentrations of the methanol extract from the marine sponge, Geodia cydonium, on normal human breast epithelial cells (MCF-10A) and human breast cancer cells (MCF-7). Our results show that this extract has no cytotoxic effects on both cell lines whereas it induces a decrease in levels of VEGF and five proinflammatory cytokines (CCL2, CXCL8, CXCL10, IFN-γ, and TNF-α) only in MCF-7 cells in a dose-dependent manner, thereby indicating an anti-inflammatory effect. Moreover, interactomic analysis suggests that all six cytokines are involved in a network and are connected with some HUB nodes such as NF-kB subunits and ESR1 (estrogen receptor 1). We also report a decrease in the expression of two NFKB1 and c-Rel subunits by RT-qPCR experiments only in MCF-7 cells after extract treatment, confirming NF-kB inactivation. These data highlight the potential of G. cydonium for future drug discovery against major diseases, such as breast cancer.