Potentiality of Bacillus subtilis as biocontrol agent for management of anthracnose disease of chilli caused by Colletotrichum gloeosporioides OGC1.
Potentiality of Bacillus subtilis as biocontrol agent for management of anthracnose disease of chilli caused by Colletotrichum gloeosporioides OGC1.
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DOI：
10.1007/s13205-013-0134-4
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发表时间：
2014-04
期刊：
影响因子：
2.8
通讯作者：
Srividya S
中科院分区：
文献类型：
--
作者：
Ashwini N;Srividya S
从辣椒根际分离得到的土壤细菌枯草芽孢杆菌对炭疽病菌gloeosporioides OGC1具有较高的拮抗活性。双板法观察到0.5 ~ 1 cm的明显抑制区。显微镜观察显示菌丝有明显的裂解和细胞壁的降解。在双重液体培养中，枯草芽孢杆菌菌株在干重方面对gloeosporioides的抑制高达100%。该菌株在含有0.5%胶体甲壳素的几丁质琼脂培养基上也产生了清晰的晕区，表明它分泌几丁质酶。菌株还产生其他酵母菌酵解酶-葡聚糖酶和纤维素酶，酵母细胞壁葡聚糖（YCW 0.1% v/v）和羧甲基纤维素（CMC 0.1% v/v）的水解区表明。在液体培养中，菌株表现出相当水平的几丁质酶、葡聚糖酶和纤维素酶活性以及以gloeosporioides OGC1菌丝为底物的水解活性。研究了枯草芽孢杆菌在体外抑制真菌生长的作用，并与该菌株的紫外突变体进行了比较，该突变体既缺乏拮抗活性，也缺乏水解活性。通过热失活（70-100℃）、胰蛋白酶和TCA处理粗酶提取物，进一步证实了真菌酵母酶介导的枯草芽孢杆菌拮抗作用。用芽孢杆菌培养辣椒种子后，其萌发率与未处理辣椒种子相似，达到100%。与单独接种病原菌的种子（77.5%）相比，将病原菌与芽孢杆菌共接种处理的种子的发病率降低了65%。
A soil bacterium, Bacillus subtilis, isolated from the rhizosphere of Chilli, showed high antagonistic activity against Colletotrichum gloeosporioides OGC1. A clear inhibition zone of 0.5–1 cm was observed in dual plate assay. Microscopic observations showed a clear hyphal lysis and degradation of fungal cell wall. In dual liquid cultures, the B. subtilis strain inhibited the C. gloeosporioides up to 100 % in terms of dry weight. This strain also produced a clear halo region on chitin agar medium plates containing 0.5 % colloidal chitin, indicating that it excretes chitinase. The strain also produced other mycolytic enzymes—glucanase and cellulase, demonstrated by a clear zone of hydrolysis of yeast cell wall glucan (YCW 0.1 % v/v) and carboxymethylcellulose (CMC 0.1 % v/v). In liquid cultures, the strain showed appreciable levels of chitinase, glucanase and cellulase activities and hydrolytic activity with C. gloeosporioides OGC1 mycelia as the substrate. The role of the B. subtilis strain in suppressing the fungal growth in vitro was studied in comparison with a UV mutant of that strain, which lacked both antagonistic and hydrolytic activity. The mycolytic enzyme mediated antagonism of B. subtilis was further demonstrated by heat inactivation (70–100 °C), treatment with trypsin and TCA of the crude enzyme extract which lacked antifungal property also. Treatment of the chilli seeds with Bacillus sp. culture showed 100 % germination index similar to the untreated seeds. The treatment of the seed with co-inoculation of the pathogen with Bacillus sp. culture showed 65 % reduction in disease incidence by the treatment as compared to the seed treated with pathogen alone (77.5 %).
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影响因子：
2.1
作者：
BROEKAERT, WF;TERRAS, FRG;VANDERLEYDEN, J
通讯作者：
VANDERLEYDEN, J
影响因子：
4.9
作者：
CHET, I;ORDENTLICH, A;OPPENHEIM, A
通讯作者：
OPPENHEIM, A
影响因子：
4.8
作者：
CHEN, MH;SHEN, ZM;LIPKE, PN
通讯作者：
LIPKE, PN
DOI：
10.1139/b76-039
发表时间：
1976-01-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
HUANG, HC;HOES, JA
通讯作者：
HOES, JA
影响因子：
2.8
作者：
Abdullah, Mansour T.;Ali, Nida Y.;Suleman, Patrice
通讯作者：
Suleman, Patrice