Identification of gene clusters associated with fusaric acid, fusarin, and perithecial pigment production in Fusarium verticillioides
Identification of gene clusters associated with fusaric acid, fusarin, and perithecial pigment production in Fusarium verticillioides
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DOI：
10.1016/j.fgb.2012.05.010
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发表时间：
2012-07-01
影响因子：
3
通讯作者：
Proctor, Robert H.
中科院分区：
文献类型：
--
作者：
Brown, Daren W.;Butchko, Robert A. E.;Proctor, Robert H.
镰刀菌属因其引起作物病害和产生霉菌毒素的能力而受到农业生产和食品/饲料安全的关注。了解霉菌毒素和其他次生代谢物 (SM) 产生的遗传基础有可能限制作物病害和霉菌毒素污染。在真菌中，SM 生物合成基因通常在共表达基因簇中彼此相邻。这样的簇通常包括负责合成初始化学物质的核心基因，以及负责化学修饰、运输和/或调节的几个基因。轮枝镰刀菌是玉米最常见的病原体之一，并产生多种令人关注的 SM。在这里，我们采用全基因组表达分析，并利用聚酮合酶（PKS）基因（一种常见的簇核心基因）的现有知识，鉴定了 F. verticillioides 中三个新的共表达基因簇。 PKS 基因的功能分析将这些簇与三种已知的镰刀菌 SM、有性子实体（子囊壳）中的紫色素以及霉菌毒素镰刀菌素 C 和镰刀菌酸的产生联系起来。结果表明，对源自诱导差异基因表达的培养条件的RNA进行微阵列分析可以成为鉴定SM生物合成基因簇的有效工具。由爱思唯尔公司出版
The genus Fusarium is of concern to agricultural production and food/feed safety because of its ability to cause crop disease and to produce mycotoxins. Understanding the genetic basis for production of mycotoxins and other secondary metabolites (SMs) has the potential to limit crop disease and mycotoxin contamination. In fungi, SM biosynthetic genes are typically located adjacent to one another in clusters of co-expressed genes. Such clusters typically include a core gene, responsible for synthesis of an initial chemical, and several genes responsible for chemical modifications, transport, and/or regulation. Fusarium verticillioides is one of the most common pathogens of maize and produces a variety of SMs of concern. Here, we employed whole genome expression analysis and utilized existing knowledge of polyketide synthase (PKS) genes, a common cluster core gene, to identify three novel clusters of co-expressed genes in F. verticillioides. Functional analysis of the PKS genes linked the clusters to production of three known Fusarium SMs, a violet pigment in sexual fruiting bodies (perithecia) and the mycotoxins fusarin C and fusaric acid. The results indicate that microarray analysis of RNA derived from culture conditions that induce differential gene expression can be an effective tool for identifying SM biosynthetic gene clusters. Published by Elsevier Inc.