Prevalence and Molecular Epidemiology of Staphylococcus aureus among Residents of Seven Nursing Homes in Shanghai.
Prevalence and Molecular Epidemiology of Staphylococcus aureus among Residents of Seven Nursing Homes in Shanghai.
复制标题
上海市七家养老院居民金黄色葡萄球菌患病率及分子流行病学分析
DOI：
10.1371/journal.pone.0137593
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Han LZ
中科院分区：
文献类型：
--
作者：
Zhang J;Gu FF;Zhao SY;Xiao SZ;Wang YC;Guo XK;Ni YX;Han LZ
养老院的居民是金黄色葡萄球菌和耐甲氧西林葡萄球菌的潜在宿主。金黄色葡萄球菌（MRSA）。据我们所知，到目前为止，还没有流行病学资料描述的流行病学和分子特征的S。金黄色葡萄球菌在养老院居民在上海，中国。方法选取7个社区卫生服务机构的491名居民作为研究对象。共采集鼻拭子491份，腋窝拭子487份，皮肤拭子119份。S.根据抗菌药物敏感性分析、毒素基因流行率、多位点序列分型（MLST）、spa和SCCmec分型对从拭子中分离和鉴定的金黄色葡萄球菌进行了表征。结果491名居民中，S. 109名居民从90份鼻拭子（90/491，18.3%）、29份腋窝拭子（29/487，6.0%）和22份皮肤拭子（22/119，18.5%）中分离出金黄色葡萄球菌。从52名居民的41名鼻带菌者、15名腋带菌者和12名皮肤带菌者中检出68株MRSA。S.金黄色葡萄球菌和MRSA的定植率分别为22.2%和10.6%。10名居民提出了S。金黄色葡萄球菌和12名居民提出了S。金黄色葡萄球菌在收集的三种样品类型中的两种中。分子生物学分析表明，CC 1（29.1%）是本研究中的优势克隆，其次是CC 398（19.9%），CC 188（13.5%）和CC 5（12.8%）。最常见的spa类型是t127（22.0%），其次是t14383（12.8%）和t002（10.6%）。结论S.金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌定植在上海的养老院居民。CC 1是最常见的克隆复合体，t127是NH居民中最常见的SPA类型。这些数据为今后监测沙门氏菌提供了重要的基线。金黄色葡萄球菌在上海和中国其他高度城市化地区的NHS。在国家卫生机构中，必须高度重视感染控制策略的实施，以对抗MRSA和甲氧西林敏感的S。金黄色葡萄球菌（MSSA）。
Background Residents in nursing homes (NHs) always represent potential reservoirs for Staphylococcus aureus and methicillin-resistant S. aureus (MRSA). To our knowledge, there is no epidemiological information up till now that describes the prevalence and molecular characteristics of S. aureus in nursing home residents in Shanghai, China. Methods Four hundred and ninety-one unique residents from 7 NHs were enrolled in this study. Specimens were collected among these residents including 491 nasal swabs, 487 axillary swabs and 119 skin swabs. S. aureus isolated and identified from the swabs was characterized according to antimicrobial susceptibility profiling, toxin gene prevalence, and multilocus sequence typing (MLST), spa and SCCmec typing. Results Among the 491 residents screened, S. aureus was isolated in 109 residents from 90 nasal swabs (90/491, 18.3%), 29 axillary swabs (29/487, 6.0%), and 22 skin swabs (22/119, 18.5%). Sixty-eight MRSA isolates were detected in 52 residents from 41 nasal carriers, 15 axillary carriers and 12 skin carriers. The overall prevalence rate of S. aureus and MRSA colonization was 22.2% and 10.6% respectively. Ten residents presented S. aureus in all three sample types and 12 residents presented S. aureus in two of the three sample types collected. Molecular analysis revealed CC1 (29.1%) to be the dominant clone in this study, followed by CC398 (19.9%), CC188 (13.5%) and CC5 (12.8%). The most common spa type was t127 (22.0%), followed by t14383 (12.8%) and t002 (10.6%). Conclusions A high prevalence of S. aureus and MRSA colonization was revealed in nursing home residents in Shanghai. CC1 was the most common clonal complex and t127 was the most common spa type among NH residents. The data provides an important baseline for future surveillance of S. aureus in NHs in Shanghai and other highly urbanized regions in China. Implementation of infection control strategies must be given high priority in NHs to fight such high prevalence of both MRSA and methicillin-susceptible S. aureus (MSSA).