Profound underestimation of glucose uptake by [18F]2-deoxy-2-fluoroglucose in reperfused rat heart muscle
Profound underestimation of glucose uptake by [18F]2-deoxy-2-fluoroglucose in reperfused rat heart muscle
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DOI：
10.1161/01.cir.97.24.2454
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发表时间：
1998-06-23
期刊：
影响因子：
37.8
通讯作者：
Taegtmeyer, H
中科院分区：
文献类型：
--
作者：
Doenst, T;Taegtmeyer, H
背景-[F-18]2-脱氧-2-氟葡萄糖（FDG）被广泛用作缺血心肌葡萄糖摄取的示踪剂。方法和结果：用Krebs-Henseleit缓冲液（仅含葡萄糖5 mmol/L）（组1）或葡萄糖5 mmol/L+油酸0.4 mmol/L（组2，进食;组3，禁食）灌流离体工作大鼠心脏，观察低流量缺血和再灌注对示踪剂/示踪剂比值（跳跃常数，LC）的影响。同时用[2-H-3]葡萄糖和FDG测定动态葡萄糖摄取。20分钟后，冠状动脉血流减少75%，持续30分钟，然后在最后20分钟恢复到对照条件。测定了细胞溶质和线粒体组分以及组织代谢产物中的己糖激酶活性。当葡萄糖是唯一底物时，葡萄糖摄取速率最高。葡萄糖摄取、FDG摄取和LC仅在组3中在缺血期间增加。在缺血期间，组1和组2的这些参数没有变化。在第2和第3组中，FDG摄取随着再灌注而显著降低，并且LC显著下降（从>1.0下降到> 1.0）。
Background-[F-18]2-deoxy-2-fluoroglucose (FDG) is widely used as a tracer for glucose uptake in ischemic heart muscle. We tested the effects of low-flow ischemia and reperfusion on the ratio of tracer/tracee (jumped constant, LC).Methods and Results-Isolated working rat hearts were perfused with Krebs-Henseleit buffer containing only glucose 5 mmol/L (group 1) or glucose 5 mmol/L plus oleate 0.4 mmol/L (group 2, fed; group 3, fasted). Dynamic glucose uptake was measured simultaneously with [2-H-3]glucose and with FDG. After 20 minutes, coronary flow was reduced by 75% for 30 minutes before it was returned to control conditions for the final 20 minutes. Hexokinase activity in the cytosolic and mitochondrial fractions and tissue metabolites were determined, Rates of glucose uptake were highest when glucose was the only substrate. Glucose uptake, FDG uptake, and the LC increased during ischemia only in group 3. There was no change of these parameters during ischemia in groups 1 and 2. FDG uptake decreased significantly with reperfusion in groups 2 and 3, and there was a striking fall in the LC (from >1.0 to