The Arabidopsis TRM61/TRM6 complex is a bona fide tRNA N1-methyladenosine methyltransferase
The Arabidopsis TRM61/TRM6 complex is a bona fide tRNA N1-methyladenosine methyltransferase
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AtTRM61/AtTRM6 复合物是拟南芥中真正的 tRNA N1-甲基腺苷甲基转移酶
DOI：
10.1093/jxb/eraa100
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发表时间：
2020-05-30
影响因子：
6.9
通讯作者：
Yang, Wei-Cai
中科院分区：
文献类型：
--
作者：
Tang, Jun;Jia, Pengfei;Yang, Wei-Cai
tRNA分子含有最丰富的转录后修饰，对基因的正确表达和蛋白质的生物合成至关重要。腺苷58（A58）的N-1甲基化对于维持细菌、古细菌和真核生物tRNA中的引发剂甲硫氨酰-tRNA（tRNAi（Met））的稳定性至关重要。然而，尽管已经在酵母和哺乳动物中进行了研究，但仍然不清楚植物tRNA中的A58是如何被修饰并参与发育的。在这项研究中，我们确定了拟南芥中的核定位复合物AtTRM 61/AtTRM 6作为tRNA m（1）A58甲基转移酶。AtTRM 61或AtTRM 6的缺乏或缺失导致胚停滞和种子败育。在条件互补的Attrm 61/LEC 1 pro：：AtTRM 61植物中，tRNA m（1）A水平降低，这伴随着tRNAi（Met）水平的降低，表明tRNA m（1）A修饰对tRNAi（Met）稳定性的重要性。综上所述，我们的结果表明，tRNA m（1）A58修饰是必要的tRNAi（Met）的稳定性和胚胎发育所需的拟南芥。
tRNA molecules, which contain the most abundant post-transcriptional modifications, are crucial for proper gene expression and protein biosynthesis. Methylation at N-1 of adenosine 58 (A58) is critical for maintaining the stability of initiator methionyl-tRNA (tRNAi(Met)) in bacterial, archaeal, and eukaryotic tRNAs. However, although research has been conducted in yeast and mammals, it remains unclear how A58 in plant tRNAs is modified and involved in development. In this study, we identify the nucleus-localized complex AtTRM61/AtTRM6 in Arabidopsis as tRNA m(1)A58 methyltransferase. Deficiency or a lack of either AtTRM61 or AtTRM6 leads to embryo arrest and seed abortion. The tRNA m(1)A level decreases in conditionally complemented Attrm61/LEC1pro::AtTRM61 plants and this is accompanied by reduced levels of tRNAi(Met), indicating the importance of the tRNA m(1)A modification for tRNAi(Met) stability. Taken together, our results demonstrate that tRNA m(1)A58 modification is necessary for tRNAi(Met) stability and is required for embryo development in Arabidopsis.