Paeoniflorin Resists H2O2-Induced Oxidative Stress in Melanocytes by JNK/Nrf2/HO-1 Pathway
Paeoniflorin Resists H2O2-Induced Oxidative Stress in Melanocytes by JNK/Nrf2/HO-1 Pathway
复制标题
DOI：
10.3389/fphar.2020.00536
复制
发表时间：
2020-04-28
影响因子：
5.6
通讯作者：
Chen, Hong-Duo
中科院分区：
文献类型：
--
作者：
Yuan, Jinping;Lu, Yansong;Chen, Hong-Duo
芍药苷具有抗氧化等多种生物学功能。白芍总苷是白芍总苷的主要活性成分，具有促进白癜风色素沉着的作用。本研究旨在探讨PF对过氧化氢（H2 O2）诱导的黑素细胞氧化应激的影响及其可能的信号通路。结果表明，50 μ M PF预处理可显著抑制细胞凋亡，提高细胞活力，并通过增加超氧化物歧化酶（SOD）和抗氧化酶过氧化氢酶（CAT）的产生，抑制活性氧（ROS）的积累。此外，PF激活c-Jun氨基末端激酶（JNK）和核因子E2相关因子2（Nrf 2）/血红素加氧酶-1（HO-1）通路，以对抗H2 O2诱导的PIG 1和PIG 3V的氧化损伤。综上所述，我们的研究首次证明PF可能通过激活JNK/Nrf 2/HO-1信号通路对抗H2 O2诱导的黑素细胞氧化应激，提示PF在白癜风治疗中具有潜在的应用价值。
Paeoniflorin (PF) possesses multiple biological functions including anti-oxidization. PF is the major bioactive ingredient of total glycosides of paeony (TGP), which could promote re-pigmentation of vitiligo. The study was sought to investigate the effects and potential signaling pathways of PF on hydrogen peroxide (H2O2)-induced oxidative stress in melanocytes. The results showed that pretreatment with 50 mu M PF significantly inhibited cell apoptosis, enhanced cell viability, and suppressed reactive oxygen species (ROS) accumulation by enhancing the productions of superoxide dismutase (SOD) and antioxidant enzymes catalase (CAT). Furthermore, PF activated c-Jun amino terminal kinase (JNK) and the nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway to counteract H2O2-induced oxidative damage in PIG1 and PIG3V. Taken together, our study firstly demonstrates that PF resists H2O2-induced oxidative stress in melanocytes probably by activating JNK/Nrf2/HO-1 signaling, suggesting a potential therapeutic application of PF on vitiligo.