Effects of controlled micro-/nanosurfaces on osteoblast proliferation
Effects of controlled micro-/nanosurfaces on osteoblast proliferation
复制标题
DOI：
10.1002/jbm.a.36118
复制
发表时间：
2017-09-01
影响因子：
4.9
通讯作者：
Nagai, Akiko
中科院分区：
文献类型：
--
作者：
Iwata, Natsuko;Nozaki, Kosuke;Nagai, Akiko
模拟天然骨结构的微/纳米结构植入物表面预计对成骨细胞增殖具有积极影响。在这项研究中，以澄清微/纳米钛表面上的最佳微观结构尺度，高度控制的微观结构表面制作与二氧化钛纳米管氧化物层喷砂处理和电化学阳极氧化。平均表面粗糙度（R-a）和平均宽度的轮廓元素（R-Sm）的喷砂介质的大小成比例增加。所有样品在阳极氧化和退火后的晶体结构和润湿性相似。MG 63成骨样骨肉瘤细胞在孵育24 h后，在较小的微结构表面上显示出减少的铺展面积。包括细胞计数和细胞周期分析在内的细胞增殖试验表明，具有较小微观结构（R-a=0.6 μ m，R-Sm=11.1 μ m）的微/纳米表面具有最积极的效果。这一结果表明，较小的显微结构的分层表面增强细胞增殖，尽管相似的化学成分和润湿性。(C)2017年Wiley Periodicals，Inc.
Micro-/nanostructured implant surfaces mimicking natural bone architecture are expected to have positive effects on osteoblast proliferation. In this study, to clarify the optimal microstructure scale on micro-/nano-titanium surfaces, highly controlled microstructure surfaces were fabricated with a titania nanotube oxide layer by sandblast treatment and electro-chemical anodization. Average surface roughness (R-a) and mean width of the profile elements (R-Sm) increased in proportion to the size of the blasting media. The crystalline structure and wettability were similar for all samples after anodizing and annealing. MG63 osteoblast-like osteosarcoma cells showed reduced spreading area on smaller microstructure surfaces after incubation for 24 h. Cell proliferation assays, including cell counting and cell cycle analyses, showed that micro-/nanosurfaces with smaller microstructures (R-a=0.6 mu m, R-Sm=11.1 mu m) had the most positive effect. This result indicated that smaller microstructures of hierarchical surfaces enhanced cell proliferation, despite similar chemical compositions and wettabilities. (C) 2017 Wiley Periodicals, Inc.