A novel role for twist-1 in pulp homeostasis
A novel role for twist-1 in pulp homeostasis
复制标题
DOI：
10.1177/154405910708601007
复制
发表时间：
2007-10-01
影响因子：
7.6
通讯作者：
D'Souza, R. N.
中科院分区：
文献类型：
--
作者：
Galler, K. M.;Yasue, A.;D'Souza, R. N.
维持成牙本质细胞前体细胞在牙髓中平衡的分子机制尚不清楚。在这里，我们测试了牙髓中的动态平衡是否受到Twist-1的调节，Twist-1是一种核蛋白，在成骨细胞分化过程中与Runx2配对。我们对Twist-1(+/-)小鼠的分析显示，表型变化包括牙本质基质形成更早开始，碱性磷酸酶活性增加，以及牙髓中的结石。RT-PCR分析显示Twist-1在包括牙髓在内的几个成年器官中都有表达。与牙髓结石相关的血管周围细胞中，Twist-1水平的降低导致I型胶原和Dspp基因表达水平升高。在Twist-1和Runx2基因杂合子失活的小鼠中，牙髓结石的表型似乎完全被挽救了。这些发现提示Twist-1在抑制成牙本质细胞分化中起关键作用，从而维持牙髓的动态平衡。此外，Twist-1在牙髓中的功能依赖于它与Runx2的相互作用。
The molecular mechanisms that maintain the equilibrium of odontoblast progenitor cells in dental pulp are unknown. Here we tested whether homeostasis in dental pulp is modulated by Twist-1, a nuclear protein that partners with Runx2 during osteoblast differentiation. Our analysis of Twist-1(+/-) mice revealed phenotypic changes that involved an earlier onset of dentin matrix formation, increased alkaline phosphatase activity, and pulp stones within the pulp. RT-PCR analyses revealed Twist-1 expression in several adult organs, including pulp. Decreased levels of Twist-1 led to higher levels of type I collagen and Dspp gene expression in perivascular cells associated with the pulp stones. In mice heterozygous for both Twist-1 and Runx2 inactivation, the phenotype of pulp stones appeared completely rescued. These findings suggest that Twist-1 plays a key role in restraining odontoblast differ entiation, thus maintaining homeostasis in dental pulp. Furthermore, Twist-1 functions in dental pulp are dependent on its interaction with Runx2.