Increased expression of mineralocorticoid receptor in human ileum after total colectomy: immunohistochemical and immunoblotting studies.
Increased expression of mineralocorticoid receptor in human ileum after total colectomy: immunohistochemical and immunoblotting studies.
复制标题
全结肠切除术后人回肠中盐皮质激素受体表达增加：免疫组织化学和免疫印迹研究。
DOI：
10.1620/tjem.173.383
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Seiki Matsuno
中科院分区：
文献类型：
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作者：
K. Fukushima;Hironobu Sasano;I. Sasaki;H. Nagura;Y. Funayama;Seiki Matsuno
已知醛固酮通过与哺乳动物结肠中的盐皮质激素受体(MR)结合来调节跨膜离子转运和水吸收。在全结肠切除术的患者中，醛固酮可能在增加残留回肠的水分吸收中起作用。因此，我们研究了全结肠切除患者残留回肠粘膜中MR的免疫定位，以研究醛固酮在这些患者水和钠吸收中的作用。以抗盐皮质激素受体融合蛋白多克隆抗体为一抗，以Biotinstrettavidin免疫组织化学染色系统，对7例全结肠切除患者和5例癌后升结肠切除的正常回肠组织进行了MR免疫组织化学定位。同时进行免疫印迹检测。全结肠切除患者回肠吸收细胞胞浆和胞核均有MR免疫阳性反应，而正常回肠未见MR免疫反应阳性反应。免疫印迹显示与盐皮质激素受体相对应的免疫反应产物约为100 kDa。醛固酮被认为作用于全结肠切除后的回肠黏膜，全结肠切除后回肠粘膜对醛固酮依赖的钠转运和水分吸收可能加速，这与这些患者术后观察到的大便容量减少是一致的。结论：盐皮质激素受体在全结肠切除患者的肠道适应中可能起重要作用。
Aldosterone is known to regulate transmembrane ion transport and water absorption through its binding to mineralocorticoid receptor (MR) in the mammalian colon. A possible roles of aldosterone has been suggested in increased water absorption from remnant ileum in the patients with total colectomy. We, therefore, studied immunolocalization of MR in remnant ileal mucosa in the patients with total colectomy in order to study the role of aldosterone in water and sodium absorption in these patients. Immunohistochemical localization of MR was performed in ileal mucosa of 7 patients with total colectomy and 5 cases of normal ileum obtained from the resection of ascending colon due to carcinoma by using a polyclonal antibody raised against a mineralocorticoid receptor fusion protein as a primary antibody and a biotinstreptavidin system for immunostaining. Immunoblotting was also performed. Positive MR immunoreactivity was observed in both cytoplasm and nucleus of absorptive cells of ileum of total colectomy patients but not in control normal ileum. Immunoblotting revealed the presence of an approximately 100 kDa immunoreactive product corresponding to mineralocorticoid receptor. Aldosterone is considered to act on ileal mucosa following total colectomy and the aldosterone dependent sodium transport and water absorption may be accelerated in ileal mucosa after total colectomy, which is consistent with the postoperative decrease in the stool volume observed in these patients. In conclusion, increased expression of the mineralocorticoid receptor in remnant ileum may play an important role in intestinal adaptation in total colectomized patients.
Hiroshi Nagura：“人体肠道中携带 T 细胞受体的淋巴细胞的免疫组织化学特征、分布和超微结构。”
DOI：
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发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
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影响因子：
3.2
作者：
MCLEAN, IW;NAKANE, PK
通讯作者：
NAKANE, PK